Single-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods: Volume 582

ISBN-13: 9780128093108 / Angielski / Twarda / 2017 / 484 str.

Maria Spies; Yann R. Chemla

Opis

Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers research methods in single-molecule enzymology
• Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion

Recenzje

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience

Spis treści

1. How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp J. Sung, K.I. Mortensen, J.A. Spudich and H. Flyvbjerg 2. Studying the Mechanochemistry of Processive Cytoskeletal Motors with an Optical Trap V. Belyy and A. Yildiz 3. Single-Molecule Optical Trapping Techniques to Study Molecular Mechanisms of a Replisome B. Sun and M.D. Wang 4. Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy M. H. Shabestari, A.E.C. Meijering, W.H. Roos, G.J. L. Wuite and E.J.G. Peterman 5. Direct Visualization of Helicase Dynamics Using Fluorescence Localization and Optical Trapping C.-T. Lin and T. Ha 6. High-Resolution Optical Tweezers Combined with Single-Molecule Confocal Microscopy K.D. Whitley, M.J. Comstock and Y.R. Chemla# 7. Integrating Optical Tweezers, DNA Tightropes and Single Molecule Fluorescence Imaging: Pitfalls and Traps J. Wang, J.T. Barnett1, M.R. Pollard and N.M. Kad 8. Single-Stranded DNA Curtains for Studying Homologous Recombination C.J. Ma, J.B. Steinfeld and E.C. Greene 9. Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of  DNA Mismatch Repair M.W. Brown, A.D.L. Torre and I.J. Finkelstein 10. Single-Molecule Insight into Target Recognition by CRISPR-Cas Complexes M. Rutkauskas, A. Krivoy, M. Szczelkun, C. Rouillon and R. Seidel 11. Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules C. Duboc, J. Fan, E.T. Graves and T.R. Strick 12. Measuring Force Induced Dissociation Kinetics of Protein Complexes Using Single Molecule Atomic Force Microscopy K. Manibog, C.-F. Yen and S. Sivasankar 13. Improved Force Spectroscopy Using Focused-Ion-Beam Modified Cantilevers J.K. Faulk, D.T. Edwards, M.S. Bull and T.T. Perkins 14. Single-Molecule Characterization of DNA-Protein Interactions Using Nanopore Biosensors A.H. Squires, T. Gilboa, C. Torfstein, N. Varongchayakul and A. Meller 15. Subangstrom Measurements of Enzyme Function using a Biological Nanopore, SPRNT A.H. Laszlo, I.M. Derrrington and J.H. Gundlach 16. Multiplexed, Tethered Particle Microscopy for Studies of  DNA-Enzyme Dynamics S. Ucuncuoglu, D.A. Schneider, E.R. Weeks, D. Dunlap and L. Finzi

O autorze

Graduate of Peter the Great St. Petersburg Polytechnic University, Russia (1996 MS diploma with honors (equivalent of cum laude) in physics/biophysics) and Osaka University, Japan (2000 PhD in biological sciences), Dr. Maria Spies is an Associate Professor of Biochemistry at the University of Iowa Carver College of Medicine.
Spies' research career has been focused on deciphering the intricate choreography of the molecular machines orchestrating the central steps in the homology directed DNA repair. Her doctoral research supported by the Japanese Government (MONBUSHO) Graduate Scholarship provided the first detailed biochemical characterization of archaeal recombinase RadA. In her postdoctoral work with Dr. Steve Kowalczykowski (UC Davis) supported by the American Cancer Society, Spies reconstituted at the single-molecule level the initial steps of bacterial recombination and helped to explain how this process is regulated.
Spies' laboratory at the University of Iowa emphasizes the molecular machinery of homologous recombination, how it is integrated into DNA replication, repair and recombination (the 3Rs of genome stability), and how it is misappropriated in the molecular pathways that process stalled DNA replication events and DNA breaks through highly mutagenic, genome destabilizing mechanisms. Her goal is to understand, reconstitute and manipulate an elaborate network of DNA recombination, replication and repair, and to harness this understanding for anticancer drug discovery. The Spies lab utilizes a broad spectrum of techniques from biochemical reconstitutions of the key biochemical reactions in DNA recombination, repair and replication, to structural and single-molecule analyses of the proteins and enzymes coordinating these reactions, to combined HTS/CADD campaigns targeting human DNA repair proteins.
Work in Spies Lab has been funded by the American Cancer Society (ACS), Howard Hughes Medical Institute (HHMI), and is currently supported by the National Institutes of Health (NIH). She received several prestigious awards including HHMI Early Career Scientist Award and Margaret Oakley Dayhoff Award in Biophysics. She serves on the editorial board of the Journal of Biological Chemistry, and as an academic editor of the journal Plos-ONE. She is a permanent member and a chair of the American Cancer Society "DNA mechanisms in cancer” review panel. Associate Professor of Physics and Biophysics, Department of Physics, University of Illinois at Urbana-Champaign, USA