Single Chain Fragment Variable (scFv) Against Breast Cell Line MCF-7

ISBN-13: 9783659606120 / Angielski / Miękka / 2014 / 172 str.

Mahgoub Elham

Opis

Abstract Recombinant antibody cloning and phage display technologies proved to be very efficient in producing single-chain antibodies (scFv), against antigen of interest which is MCF-7 breast cancer cell line. The main objectives of this study are to construct single chain variable fragment (scFv) towards MCF-7 breast cancer cells and to characterize scFv antibodies that interacts with MCF-7 breast cancer cells line. In the methods: phage display technologies were used to produce single-chain antibodies (scFv) against (MCF-7) breast carcinoma cell line. The starting material was the mouse B cell hybridoma line C3A8, which generates a monoclonal antibody against breast cancer cells (MCF-7). The integrated cloning, screening, and phage display system allowed to obtain a scFv sequences derived from the hybridoma C3A8 cell line. The scFv gene was then cloned into nova-blue host strain for cloning purposes and then into origami DE host strain for further characterization. The purified single-chain antibody was purified using Immobilized Metal Affinity Chromatography (IMAC).the recombinant antibody technology is a rapid approach to next generation of cancer diagnosis and therapy antibody

O autorze

She is graduated her BSc of animal science in 2000 at Alneelain university in sudan, she finished her Master degree of virology in 2005 from University Putra Malaysia at Malaysia, she spent 4 years in laboratory of molecular and cell biology as research assistant then went to sudan to finish her PhD in Molecular immunology in 2013.