Signal Transduction Immunohistochemistry: Methods and Protocols

ISBN-13: 9781071628102 / Angielski / Twarda / 2022 / 328 str.

Alexander E. Kalyuzhny

Opis

This new edition collects diverse protocols compiled by experts from different areas of research as well as by biotech researchers developing novel technologies in the area of immunohistochemistry (IHC). After a few chapters to help orient novices entering the biomedical arena, the book includes methods chapters covering multiplex IHC including fluorescence and chromogenic techniques, combination of IHC with In Situ Hybridization (ISH), wavelet transform approach for organelle tracking, transcription factors in human stem cells, differentiation of mesenchymal stem cells, 3D imaging, phenotyping of glial cells in the human brain, tissue fixation, permeabilization, and cryo-preservation, as well as other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Signal Transduction Immunohistochemistry: Methods and Protocols, Third Edition aims to arm novices and experts with vital protocols they can use either as-is or tailor them for specific experimental needs.

Spis treści

1. Top 5 Considerations When Choosing an Antibody

            Amy Archuleta and Michael D. Browning

 

2. Validating Antibody Specificities for Immunohistochemistry by Protein Blotting

            Biji T. Kurien and R. Hal Scofield

 

3. Recent Advances in Chromogens for Immunohistochemistry

            Bipin Gupta, George Yang, Olga Petrauskene, and Marc Key

 

4. Overview of Imaging Techniques in Immunohistochemistry

            Gerald Sedgewick

 

5. Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells

            Pravin J. Mishra and Debabrata Banerjee

 

6. Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments

            Hemant K. Mishra

 

7. Labeling of Phospho-Specific Antibodies with oYo-Link® Epitope Tags for Multiplex Immunostaining

            Jingwen Niu, Jodi Hagen, Feifan, Yu, Alexander E. Kalyuzhny, and Andrew Tsourkas

 

8. Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species

            Matthew Levin, Zohreh AkhavanAghdam, and David Schwartz

 

9. 3D Imaging for Cleared Tissues and Thicker Samples on Confocal and Light-Sheet Microscopes

            Sharla L. White, Amy T. Lam, and Hannah D. Buck

 

10. Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research

            Simeon C. Daeschler and Gregory H. Borschel

 

11. Protocol and Software for Automated Detection of Lysosome Active “Runs” and “Flights” with Wavelet Transform Approach

            Kristiana Kandere-Grzybowska, Konstantin Polev, Diana V. Kolygina, and Bartosz A. Grzybowski

 

12. Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample Processing

            Christine Goetz, Chris Hammerbeck, Kristina Boss, Ryan Peterson, and Jody Bonnevier

 

13. In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-Detection of Phosphorylated EGFR in A431 Cultured Cells

            Jodi Hagen, Ashley Oliver, and Alexander E. Kalyuzhny

 

14. In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-Detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue

            Ashley Oliver, Jodi Hagen, Sue Yang, and Alexander E. Kalyuzhny

 

15. Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry

            Wenfei Kang, Anthony Santella, Eric Rosiek, Maria Pulina, Eric Chan, Ning Fan, Murray J. Tipping, Afsar Barlas, Yevgeniy Romin, and Katia Manova-Todorova

 

16. Transcription Factor-Mediated Differentiation of Motor Neurons from Human Pluripotent Stem Cells

            Junjiao Wu and Yu Tang

 

17. Immunohistochemical Techniques for Phosphoproteins

            Berenice L. Sanz Ressel and Alfredo A. Molinolo

 

18. Visualizing Cofilin-Actin Filaments by Immunofluorescence and CryoEM: Essential Steps for Observing Cofilactin in Cells

            Laurie S. Minamide, Ryan Hylton, Matthew Swulius, and James R. Bamburg

 

19. Cyclic Multiplex Fluorescent Immunohistochemistry Protocol to Phenotype Glial Cells in Human Formalin-Fixed Paraffin-Embedded Brain Sections

            Clara Muñoz-Castro, Ayush Noori, Bradley T. Hyman, and Alberto Serrano-Pozo

 

20. Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry

            Lokman Pang, Matthias Ernst, and Jennifer Huynh

 

21. Highly Multiplexed Immuno-Fluorescence Imaging for Quantitative Spatial Analysis in Tissue Samples with ChipCytometry^TM

            Thomas D. Campbell, Arne Christians, and Madison A. Tyler