Septins: Volume 136

ISBN-13: 9780128039984 / Angielski / Twarda / 2016 / 382 str.

Amy S. Gladfelter

Opis

Septins provides established septin and molecular and developmental biologists and researchers new to the field with proven, state-of-art techniques and relevant historical background and theory to aid efficient design and effective implementation of experimental methodologies. Topics include the purification of septin proteins from diverse systems, their visualization in live cells, and their analysis by a variety of cutting-edge microscopy approaches.

• Provides the latest information on septins
• Includes both established and new technologies
• Brings together specialists from the field who contribute their expertise

Recenzje

Praise for the Series: "The series is invaluable for workers at all levels of cell biology." --Nature

Spis treści

1. The Non-Opisthokont Septins: How Many There Are, How Little We Know About Them, and How We Might Learn MoreMasayuki Onishi and John R. Pringle2. Preparing Recombinant Yeast Septins and Their Analysis by Electron MicroscopyAurélie Bertin and Eva Nogales3. A FRET-Based Method for Monitoring Septin Polymerization and Binding of Septin-Associated ProteinsElizabeth A. Booth and Jeremy Thorner4. In Vitro Reconstitution of Septin Assemblies on Supported Lipid BilayersAndrew A. Bridges and Amy S. Gladfelter5. Visualization of In Vivo Septin Ultrastructures by Platinum Replica Electron MicroscopyKaty Ong, Tatyana Svitkina and Erfei Bi6. Assays for Genetic Dissection of Septin Filament Assembly in Yeast, From De Novo Folding Through PolymerizationMichael A. McMurray7. Investigation of Septins Using Infection by Bacterial PathogensSina Krokowski and Serge Mostowy8. In Vivo Analysis of Septin Heteropolymer Rods and Higher-Order Structures in Filamentous FungiAmy Smith and Michelle Momany9. Live Cell Imaging of Septin Dynamics in Ustilago maydisSebastian Baumann, Sabrina Zander, Stefanie Weidtkamp-Peters and Michael Feldbrügge10. Ashbya gossypii as a Model System to Study Septin Organization by Single-Molecule Localization MicroscopyCharlotte Kaplan, Chenlu Yu and Helge Ewers11. Visualizing Septins in Early Drosophila EmbryosManos Mavrakis12. Purification of Recombinant Human and Drosophila Septin Hexamers for TIRF Assays of Actin-Septin Filament AssemblyManos Mavrakis, Feng-Ching Tsai and Gijsje H. Koenderink13. Investigation of Septin Biology In Vivo Using ZebrafishAlexandra Willis, Maria Mazon Moya and Serge Mostowy14. Fluorescence Microscopy of Actin- and Microtubule-Associated Septins in Mammalian CellsElias T. Spiliotis, Eva P. Karasmanis and Lee Dolat15. Immunofluorescent Staining of Septins in Primary CiliaMoshe S. Kim, Carol D. Froese, Hong Xie and William S. Trimble16. Methods for Immunoblot Detection and High-Resolution Subcellular Mapping of Septin Subunits in Mammalian Nervous SystemsLaxmi Kumar Parajuli, Natsumi Ageta-Ishihara, Hiroshi Ageta, Yugo Fukazawa and Makoto Kinoshita17. Visualizing Septin and Cell Dynamics in Mammalian Brain SlicesHidenori Ito, Rika Morishita, Hidenori Tabata and Koh-ichi Nagata18. Small Molecule Perturbations of SeptinsLydia R. Heasley and Michael A. McMurray19. Septin Crystallization for Structural AnalysisNapoleão Fonseca Valadares and Richard Charles Garratt