Sepsis: Methods and Protocols

ISBN-13: 9781071614907 / Angielski / Miękka / 2022

Walker, Wendy E.

Opis

This detailed volume presents a variety of animal models that are commonly used to study sepsis and some key procedures to measure specific disease outcomes. The chapters describe well-established surgical and nonsurgical rodent models of sepsis, presented by experts in the field. In addition, the book includes protocols for burn injury and sepsis, modeling the mouse intensive care unit (MICU), and the development of humanized mice, which may be useful tools to increase the translational potential of rodent sepsis research. There is a chapter discussing the use of biomarkers for sepsis diagnosis and prognosis in humans and mice, as well as chapters describing specific methods for biomarker measurement, and the use of analgesics and humane endpoints in rodent sepsis research. Finally, agent-based computational modeling is presented as a valuable complementary approach to study sepsis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Sepsis: Methods and Protocols serves as an ideal guide for researchers working toward improving the identification and treatment of septic patients.

Spis treści

1. Cecal Ligation and Puncture

            Susanne Drechsler and Marcin Osuchowski

 

2. Colon Ascendens Stent Peritonitis (CASP)

            Anna Herminghaus and Olaf Picker

 

3. Induction of Sepsis via Fibrin Clot Implantation

            Sailaja Ghanta, Min-Young Kwon, and Mark A. Perrella

 

4. Cecal Slurry Injection in Neonatal and Adult Mice

            Jaimar C. Rincon, Philip A. Efron, Lyle L. Moldawer, and Shawn D. Larson

 

5. Injection of Escherichia coli to Induce Sepsis

            Xian-Hui He, Dong-Yun Ouyang, and Li-Hui Xu

 

6. A Mouse Model of Pseudomonas aeruginosa Pneumonia

            Brian W. LeBlanc and Craig T. Lefort

 

7. A Mouse Model of Candidiasis

            Pilar Fajardo, Ana Cuenda, and Juan José Sanz-Ezquerro

 

8. Francisella tularensis Infection of Mice as a Model of Sepsis

            Charles T. Spencer, Mireya G. Ramos Muniz, Nicole R. Setzu, and Michelle A. Sanchez

 

9. A Mouse Model of Necrotizing Enterocolitis

            Belgacem Mihi, Wyatt E. Lanik, Qingqing Gong, and Misty Good

 

10. A Murine Model of Full-Thickness Scald Burn Injury with Subsequent Wound and Systemic Bacterial Infection

            Antonio Hernandez, Naeem K. Patil, and Julia K. Bohannon

 

11. Mouse Intensive Care Unit (MICU)

            Tamara Merz, Sandra Kress, Michael Gröger, Peter Radermacher, and Oscar McCook

 

12. Creation of BLT Humanized Mice for Sepsis Studies

            Erica L. Heipertz and Wendy E. Walker

 

13. Scoring Sepsis Severity in Mice

            Tina S. Mele

 

14. Identification of ILC2 in the Lung Using Flow Cytometry

            Hui Xu and Meihong Deng

 

15. Measurement of Intestinal Permeability During Sepsis

            Takehiko Oami and Craig M. Coopersmith

 

16. Sepsis Biomarkers

            Yachana Kataria and Daniel Remick

 

17. Detection of Blood Cell Surface Biomarkers in Septic Mice

            Dinesh G. Goswami and Wendy E. Walker

 

18. Microfluidic Chips for Sepsis Diagnosis

            Yun Zhou, Yijia Yang, and Dimitri Pappas

 

19. Analgesia and Humane Endpoints for Rodents in Sepsis Research

            Christine A. Boehm and Jean A. Nemzek

 

20. Agent-Based Modeling of Systemic Inflammation: A Pathway towards Controlling Sepsis

            Gary An and Chase Cockrell