Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems

ISBN-13: 9780367886691 / Angielski / Miękka / 2019 / 271 str.

Peter Schuck; Huaying Zhao

Opis

Building on the previous two volumes by Dr. Schuck, this new volume is devoted to the theory and practical data analysis of dynamically coupled sedimentation processes. It will be an invaluable resource for students interested in the application of analytical ultracentrifugation in the study of interacting systems.

Spis treści

Exact Description of Ideally Sedimenting Associating Systems. Phenomenology of Sedimentation of Interacting Systems. Approximate Solutions for the Coupled Lamm Equations of Reacting Systems. Sedimentation Coefficient Distributions. Nonideal Sedimentation Velocity. Practical Analysis of Interacting Systems. Numerical Solution of Coupled Systems of Lamm Equations for Interacting Systems. Effective Particle Theory for Multi-Component Systems.

O autorze

Dr. Schuck obtained his Ph.D. from the Goethe-University Frankfurt am Main, Germany, where he worked on interactions of integral proteins of the erythrocyte membrane using analytical ultracentrifugation. He received his post-doctoral research training in physical biochemistry with Dr. Allen Minton at NIDDK, and joined the Bioengineering and Physical Science Program of NCRR as a Research Fellow in 1997. He is currently a Earl Stadtman Tenure-Track Investigator and Chief of the Dynamics of Macromoleular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics at the National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health.

Dr. Zhao received her Ph.D. in Chemistry, with a specialization in protein biochemistry, before spending two years on her post-doctoral research at the University of Maryland, where she worked on the study of protein-DNA and protein-protein interactions.  Dr. Zhao joined the NIBIB as a Research Fellow in 2008.  She has been working in the intramural research’s Laboratory of Cellular Imaging and Macromolecular Biophysics, Dynamics of Macromolecular Assembly Section on development of biophysical methodology for characterizing a variety of macromolecules, including proteins, nucleic acids, polymers and nanoparticles.  Her current work has focused on applications of advanced techniques of biophysics and physical biochemistry, including sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation, isothermal titration calorimetry, fluorescence spectroscopy and surface plasmon resonance biosensing.