Sars- And Other Coronaviruses: Laboratory Protocols

ISBN-13: 9781617378157 / Angielski / Miękka / 2010 / 326 str.

Dave Cavanagh

Opis

In 2003, the word coronavirus spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Comprehensive and cutting-edge, SARS- and Other Coronaviruses Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses."

Recenzje

From the reviews:

"The serious nature of coronaviruses became well known with the discovery of the severe acute respiratory syndrome (SARS) virus which spread rapidly with life-threatening consequences in humans. ... this book describes the laboratory techniques used to study them. ... Scientists and students working in virology laboratories and, specifically, laboratories working with coronaviruses are the intended audience. ... each chapter contains a copious amount of detailed information as well as specific notes with technical advice. This is a valuable resource for scientists working with coronaviruses." (Rebecca T. Horvat, Doody's Review Service, November, 2008)

"This is volume 454 in the series Methods in Molecular Biology. ... if you work on coronaviruses, or want to enter the field, this is a must-have book at an affordable price." (Stuart Siddell, Microbiology Today, February, 2009)

Spis treści

Preface Section 1. Detection and discovery of coronaviruses 1) A pancoronavirus RT-PCR assay for detection of all known coronaviruses, Leen Vijgen*, Elien Moës, Els Keyaerts, Sandra Li, and Marc Van Ranst 2) Detection of Group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactions, Leo L. M. Poon* 3) Detection and sequence characterisation of the 3’ end of coronavirus genomes harbouring the highly conserved rna motif s2m, Christine Monceyron Jonassen* 4) RT-PCR detection of avian coronaviruses of galliform birds (chicken, turkey, pheasant) and in a parrot. Francesca Anne Culver*, Paul Britton and Dave Cavanagh. 5) Detection of group 2a coronaviruses with emphasis on bovine and wild ruminant strains: virus isolation, and detection of antibody, antigen, and nucleic acid, Mustafa Hasoksuz, Anastasia Vlasova, Linda J. Saif* 6) Detection of SARS coronavirus in humans and animals by conventional and quantitative (real time) coronavirus reverse transcription polymerase chain reactions, J. S. Malik Peiris and Leo L. M. Poon* 7) Detection of new viruses by VIDISCA: virus discovery based on cDNA-amplified fragment length polymorphism, Krzysztof Pyrc, Maarten F. Jebbink, Ben Berkhout and Lia van der Hoek* Section 2. Isolation, growth, titration and purification of coronaviruses 8) Titration of human coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay, Francine Lambert, Hélène Jacomy, Gabriel Marceau, Pierre J. Talbot* 9) The preparation of chicken tracheal organ cultures for virus isolation, propagation and titration, Brenda V. Jones* and Ruth M. Hennion 10) Isolation and Propagation of Coronaviruses in Embryonated Eggs, James S. Guy* 11) Large scale preparation of UV-inactivatedSARS coronavirus virions for vaccine antigen, Yasuko Tsunetsugu-Yokota.* Section 3. Structure of CoVs analysed by electron microscopy 12) Purification and electron cryomicroscopy of coronavirus particles, Benjamin W. Neuman*, Brian D. Adair, Mark Yeager and Michael J. Buchmeier Section 4. Expression of coronavirus proteins, and crystallization 13) Production of coronavirus non-structural proteins in soluble form for crystallization Yvonne Piotrowski, Rajesh Ponnusamy, Stephanie Glaser, Anniken Daabach, Ralf Moll, and Rolf Hilgenfeld* Section 5. Raising antibodies to coronavirus proteins 14) Generating antibodies to the gene 3 proteins of infectious bronchitis virus, Amanda R. Pendleton, and Carolyn E. Machamer* 15) Establishment and characterization of monoclonal antibodies against SARS coronavirus, Kazuo Ohnishi* 16) Production of monospecific rabbit antisera recognizing nidovirus proteins, Jessika C. Zevenhoven-Dobbe, Alfred L.M. Wassenaar, Yvonne van der Meer, and Eric J. Snijder* Section 6. Manipulating the genomes of coronaviruses 17) Manipulation of the coronavirus genome using targeted RNA recombination with interspecies chimeric coronaviruses, Cornelis A.M. de Haan*, Bert Jan Haijema, Paul S. Masters, and Peter J.M. Rottier 18) Generation of recombinant coronaviruses using vaccinia virus as cloning the vector, and stable cell lines containing coronaviral replicon RNAs Klara K. Eriksson, Divine Makia, and Volker Thiel* 19) Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses, Maria Armesto, Rosa Casais, Dave Cavanagh, and Paul Britton*. 20) Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes, Fernando Almazán, Carmen Galán and Luis Enjuanes* 21)

Blurb

In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, SARS- and Other Coronaviruses: Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.