Salamanders

ISBN-13: 9781071626610 / Angielski / Miękka / 2023

Opis

This volume provides a comprehensive reference for researchers aiming to bring new techniques and approaches to their scientific research using urodeles. Chapters are authored by leaders in the field and meant to guide readers through laboratory colony husbandry, traditional molecular techniques, experimental manipulation and surgeries, bioinformatics and genomics, transgenics and lineage-tracing, and physiological and organismal techniques. In addition to laboratory methods, this volume highlights techniques developed for field studies and work with wild-caught animals. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Methods in Salamander Research aims to be a practical guide for researchers interested in deploying new  methodologies in their lab or in the field. 

Spis treści

1          Salamanders as key models for development and regeneration research

                        Malcolm Maden

 

Part I:  LABORATORY COLONY HUSBANDRY

 

            2          Establishing a new research axolotl colony

                        Anastasia S. Yandulskaya and James R. Monaghan

 

            3          Health monitoring for laboratory salamanders

                        Marcus J. Crim and Marcia L. Hart

 

4          Husbandry, captive breeding and field survey of Chinese giant salamander (Andrias davidianus)

Wansheng Jiang, Haifeng Tian, and Lu Zhang

 

Part II: TRADITIONAL MOLECULAR TECHNIQUES

 

            5          Whole mount in situ hybridization (WISH) for salamander embryos

and larvae

Sruthi Purushothaman and Ashley W. Seifert

 

6          Hybridization chain reaction fluorescence in situ hybridization

(HCR-FISH) in Ambystoma mexicanum tissue

Alex M. Lovely, Timothy J. Duerr, David F. Stein, Evan T. Mun, and James R. Monaghan

 

            7          Ethyl cinnamate-based tissue clearing strategies

                        Wouter Masselink and Elly M. Tanaka

 

            8          Induction and characterization of cellular senescence in salamanders

                        Qinghao Yu, Hannah E. Walters, and Maximina H. Yun

 

            9          Methods for studying appendicular skeletal biology in axolotls

                        Camilo Riquelme-Guzmán and Tatiana Sandoval-Guzmán

 

10        Fluorescence in situ hybridization of DNA-probes on mitotic

chromosomes of the Mexican axolotl

Melissa Keinath and Vladimir Timoshevskiy

 

            11        The use of small molecules to dissect developmental and regenerative

processes in axolotls

Stéphane Roy

            12        COMET Assay for detection of DNA damage during axolotl tail

regeneration

Belfran Carbonell, Jennifer Álvarez, Gloria A. Santa-González and  

Jean Paul Delgado

 

Part III:           EXPERIMENTAL MANIPULATIONS AND SURGERIES

 

13        In vivo and Ex vivo View of Newt Lens Regeneration

Georgios Tsissios, Anthony Sallese, Weihao Chen, Alyssa Miller, Hui Wang

and Katia Del Rio-Tsonis

 

            14        Bead implantation and delivery of exogenous growth factors

                        Rena Kashimoto, Saya Furukawa, Sakiya Yamamoto, and Akira Satoh

            15        The Accessory Limb Model regenerative assay and its derivatives

                        Michael Raymond and Catherine D. McCusker

 

            16        Embryonic Tissue and Blastema Transplantations

Maritta Schuez, Thomas Kurth, Joshua D. Currie, and Tatiana

Sandoval-Guzmán

 

            17        Retinoic acid-induced limb duplications

                        Malcolm Maden and Trey Polvadore

 

18        Isolation and characterization of peritoneal macrophages from salamanders

Anthony Sallese, Georgios Tsissios, José Raúl Pérez-Estrada, Arielle Martinez and Katia Del    Rio-Tsonis

 

Part IV: BIOINFORMATICS AND GENOMICS

 

            19        Navigation and use of custom tracks within the axolotl genome browser

                        Sergej Nowoshilow and Elly M. Tanaka

 

            20        Chromosome Conformation Capture for large genomes

                        Akane Kawaguchi and Elly Tanaka

 

Part V: TRANSGENICS AND LINEAGE-TRACING

 

            21        Axolotl transgenesis via injection of I-SceI meganuclease or Tol2

transposon system

Maritta Schuez and Tatiana Sandoval-Guzmán

            22        A practical guide for CRISPR-Cas9-induced mutations in axolotls

                        Konstantinos Sousounis, Katharine Courtemanche, Jessica L. Whited

 

            23        Applying a knock-in strategy to create reporter tagged knockout alleles in axolotls Liqun Wang, Yan-Yun Zeng, Yanmei Liu and Ji-Feng Fei

 

            24        Baculovirus production and infection in axolotls

Prayag Murawala, Catarina R. Oliveira, Helena Okulski,

Maximina H. Yun, and Elly M. Tanaka

 

            25        Cell dissociation techniques in salamanders

                        Gabriela Johnson, Nadjib Dastagir, Zachary Beal, Andrew Hart, and

James Godwin

 

Part VI :          PHYSIOLOGICAL AND ORGANISMAL TECHNIQUES

 

            26        Axolotl metabolism: measuring metabolic energy

                        Moshe Khurgel

 

            27        Artificial insemination in axolotls

                        Yuka Taniguchi-Sugiura and Elly Tanaka

 

            28        Screening salamanders for symbionts

                        Elli Vickers and Ryan Kerney

 

            29        Assessing leukocyte profiles of salamanders and other amphibians:

a herpetologists’ guide

Andrew K. Davis and John C. Maerz

Part VII: EPILOGUE

            30        Best practices to promote data utility and recycling by the salamander

community     

Garrett S. Dunlap and Nicholas D. Leigh

            31        Now that we got there, what next?

                        Elly Tanaka