SARS-CoV-2: Methods and Protocols

ISBN-13: 9781071621103 / Angielski / Twarda / 2022

Chu, Justin Jang Hann

Opis

This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, as well as bioinformatics and proteomics approaches in cellular response studies. This comprehensive collection also covers methods in immunology, animal models, antivirals and vaccine development strategies, as well as biorisk and mitigation measurements for SARS-CoV-2 research. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that is vital for success in the lab. 

Practical and timely, SARS-CoV-2: Methods and Protocols serves as an ideal guide for scientists investigating this prevalent and perilous RNA virus and the novel coronavirus disease that results from it.

Spis treści

Part I: Clinical and Diagnostic Virology

 

1. Evolution and Epidemiology of SARS-CoV-2 Virus

            Yu-Nong Gong, Kuo-Ming Lee, and Shin-Ru Shih

 

2. Molecular Epidemiology of SARS-CoV-2 by Sequencing

            Yan Yan and Qinxue Hu

 

3. Advanced Genetic Methodologies in Tracking Evolution and Spread of SARS-CoV-2

            Xuemei Yang, Ning Dong, and Sheng Chen

 

4. Antigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2:  Assessing Performance

            Vidya Keshav, Lesley Scott, Anura David, Lara Noble, Elizabeth Mayne, and Wendy Stevens

 

5. Diagnostic Method for COVID-19 Using Sugar Chain-Immobilized Nanoparticles and Saliva Specimens

            Yasuo Suda, Yasuhisa Tajima, Jun-ichiro Nishi, and Takashi Kajiya

 

6. Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay

            Alexander Wilhelm, Christiane Pallas, Rolf Marschalek, and Marek Widera

 

7. Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans

            Linda Hueston

 

Part II: Cell Culture, Virus Replication, and Cellular Responses

 

8. Propagation and Quantification of SARS-CoV-2

            Alexander S. Jureka and Christopher F. Basler

 

9. Quantification of Infectious SARS‐CoV‐2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay

            C. Korin Bullen, Stephanie L. Davis, and Monika M. Looney

 

10. One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

            Raphael Nyaruaba, Xiohong Li, Caroline Mwaliko, Faith Ogolla, Changchang Li, Lu Zhao, Hang Yang, Junping Yu, and Honping Wei

 

11. Profiling SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics

            Lucia Grenga, Duarte Gouveia, and Jean Armengaud

 

12. Detection of SARS-CoV-2 by Mass Spectrometry

            Petra Wandernoth, Katharina Kriegsmann, Jörg Kriegsmann, and Mark Kriegsmann

 

13. Bioinformatics-Based Approaches to Study Virus–Host Interactions During SARS-CoV-2 Infection

            Muhammad Saad Khan, QudsiaYousafi, Shabana Bibi, Muhammad Azhar, and Awais Ihsan

 

14. Human Nasal Epithelial Cells (hNECs) Generated by Air-Liquid Interface (ALI) Culture as a Model System for Studying the Pathogenesis of SARS-CoV-2

            Kai Sen Tan, Akshamal M. Gamage, and Jing Liu

 

Part III: Immunology and Animal Models

 

15. Animal Models of COVID-19: Nonhuman Primates

            Dhiraj Kumar Singh, Journey Cole, Ruby Escobedo, Kendra J. Alfson, Bindu Singh, Tae-Hyung Lee, Xavier Alvarez, Shashank R. Ganatra, Ricardo Carrion, Jr, and Deepak Kaushal

 

16. Animal Models of COVID-19: Transgenic Mouse Model

            Jun-Gyu Park, Paula A. Pino, Anwari Akhter, Xavier Alvarez, Jordi B. Torrelles, and Luis Martinez-Sobrido

 

17. Immunohistochemical Detection of SARS-CoV-2 Antigens, by Single and Multiple Immunohistochemistry

            Silvia Lonardi, Mattia Bugatti, Arianna Valzelli, and Fabio Facchetti

 

18. Measuring Neutralizing Antibodies to SARS-CoV-2 Using Lentiviral Spike-Pseudoviruses

            Sabari Nath Neerukonda, Russell Vassell, Carol D. Weiss, and Wei Wang

 

Part IV: Antivirals and Vaccines

 

19. Antiviral Strategies Against SARS-CoV-2: A Systems Biology Approach

            Erica T. Prates, Michael R. Garvin, Piet Jones, J. Izaak Miller, Kyle A. Sullivan, Ashley Cliff, Joao Gabriel Felipe Machado Gazolla, Manesh Shah, Angelica M. Walker, Matthew Lane, Christopher T. Rentsch, Amy Justice, Mirko Pavicic, Jonathon Romero, and Daniel Jacobson

 

20. Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test

            Sandra Westhaus and Holger F. Rabenau

 

21. Pseudovirus-Based Assays for the Measurement of Antibody-Mediated Neutralization of SARS-CoV-2

            Corey Balinsky, Vihasi Jani, Peifang Sun, Maya Williams, Gabriel Defang, and Kevin R. Porter

 

22. Cytopathic Effect (CPE)-Based Drug Screening Assay for SARS-CoV-2

            Yan Ling Ng, Chee-Keng Mok, and Justin Jang Hann Chu

 

Part V: Biorisk and Mitigation Measurements

 

23. Strengthening Biorisk Management in Research Laboratories with Security-Sensitive Biological Agents like SARS-CoV-2

            Sabai Phyu, Tessy Joseph, and Margarida Goulart

 

24. Biorisk Management for SARS-CoV-2 Research in a Biosafety Level-3 Core Facility

            Tessy Joseph, Sabai Phyu, Su Yun Se-Thoe, and Justin Jang Hann Chu

 

25. Methods of SARS-CoV-2 Inactivation

            Enyia R. Anderson, Tessa Prince, Lance Turtle, Grant L. Hughes, and Edward I. Patterson