Rhodopsin: Methods and Protocols

ISBN-13: 9781071623282 / Angielski / Twarda / 2022

Gordeliy, Valentin

Opis

This volume provides readers with the latest information on the advances made in the field of rhodopsins. The chapters in this book cover topics such as new discoveries and developments; new ways to search for rhodopsins; methods to characterize the function and structure of rhodopsins on a molecular level; nano volume high throughput in meso crystallization, and fourth generation x-ray synchrotron sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Rhodopsin: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. Microbial Rhodopsins
Valentin Gordeliy, Kirill Kovalev, Ernst Bamberg, Francisco Rodrigez-Valera, Dmitrii Zabelskii, Alexey Alekseev, Riccardo Rosselli, Ivan Gushchin, and Ivan Okhrimenko

2. Molecular Biology of Microbial Rhodopsins
Martin Engelhard

3. Rhodopsin-Based Optogenetics: Basics and Applications
Alexey Alekseev, Valentin Gordeliy, and Ernst Bamberg

4. Searching Metagenomes for New Rhodopsins
Francisco Rodriguez Valera, Alina Pushkarev, Riccardo Rosselli, and Oded Béjà

5. E. coli Expression and Purification of Microbial and Viral Rhodopsins
Taras Balandin, Dmitro Volkov, Alexey Alexeev, Kirill Kovalev, Dmitry Bratanov, and Valentin Gordeliy

6. Crystallization of Microbial Rhodopsins
Kirill Kovalev, Roman Astashkin, Valentin Gordeliy, and Vadim Cherezov

7. Crystallographic Studies of Rhodopsins: Structure and Dynamics
Marie Luise Grünbein, Gabriela Nass Kovacs, Marco Kloos, Alexander Gorel, R. Bruce Doak, Robert L. Shoeman, Thomas R.M. Barends, and Ilme Schlichting

8. Time-Resolved UV-VIS Spectroscopy of Microbial Rhodopsins
Dmytro Soloviov, Valentin Borshchevskiy, and Igor Chizhov

9. Solid-State NMR Spectroscopy on Microbial Rhodopsins
Clara Nassrin Kriebel, Johanna Becker-Baldus, and Clemens Glaubitz

10. FTIR and Raman Spectroscopy of Rhodopsins
Hideki Kandori and Yasuhisa Mizutani

11. Single Cell Resolution Optogenetics via Expression of Soma-Targeted Rhodopsins
Changyang Linghu, I-Wen Chen, Dimitrii Tanese, Valeria Zampini, and Or A. Shemesh

12. Electrophysiological Characterization of Microbial Rhodopsins Transport Properties: Electrometric and ΔpH Measurements using Planar Lipid Bilayer, Collodion Film and Fluorescent Probes Approaches
Tatiana I. Rokitskaya, Nina L. Maliar, Sergey A. Siletsky, Valentin Gordeliy, and Yuri N. Antonenko

13. Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments
Thomas Mager

14. Molecular Optimization of Rhodopsin-Based Tools for Neuroscience Applications
Lief E. Fenno, Rivka Levy, and Ofer Yizhar

15. Optogenetic Studies of Mitochondria
Kai Chen, Patrick Ernst, Xiaoguang (Margaret) Liu, and Lufang Zhou

16. In Vivo and In Vitro Characterization of Cyclase and Phosphodiesterase Rhodopsins
Yuehui Tian, Shiqiang Gao, and Georg Nagel

17. Optogenetic Control of Human Stem Cell-Derived Neurons
Rouhollah Habibey, Johannes Striebel, Kritika Sharma, and Volker Busskamp

Subject Index List…