Rhinoviruses: Methods and Protocols

ISBN-13: 9781493955336 / Angielski / Miękka / 2016 / 190 str.

David A. Jans; Reena Ghildyal

Opis

Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chronic obstructive pulmonary disease (COPD) exacerbations. The huge advancements in technical applications of biophysics and improvements in high-end imaging techniques have also had implications for HRV research among other infectious diseases. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Rhinoviruses: Methods and Protocols seeks to serve both professionals and novices with the latest technical advances in HRV research to ultimately enable the development of therapeutic approaches to combat HRV, and most importantly, its pathogenic effects in asthma exacerbation.

Spis treści

1. Classification and Evolution of Human Rhinoviruses

                Ann C. Palmenberg and James E. Gern

 

2. Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples

                Xuelin Yu and Reena Ghildyal

 

3. Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples

                Wai-Ming Lee, Kris Grindle, Rose Vrtis, Tressa Pappas, Fue Vang, Iris Lee, James E. Gern

 

4. Molecular Genotyping of Human Rhinovirus by PCR and Sanger Sequencing

                Wei Wang, Jing He, Yi Liu, Lei Xu, Wencai Guan, and Yunwen Hu

 

5. Growth of Human Rhinovirus in H1-Hela Cell Suspension Culture and Purification of Virions

                Wai-Ming Lee, Yin Chen, Wensheng Wang, and Anne Mosser

 

6. Propagation of Rhinovirus – C Strains in Differentiated Epithelial Cells of Human Airway at Air-Liquid Interface

                Shamaila Ashraf, Rebecca Brockman-Schneider, and James E. Gern

 

7. Infectivity Assays of Human Rhinovirus-A and –B Serotypes

                Wai-Ming Lee, Yin Chen, Wensheng Wang, and Anne Mosser

 

8. Application of FCS in Studies of Rhinovirus Receptor Binding and Uncoating

                Shushan Harutyunyan, Arthur Sedivy, Gottfried Köhler, Heinrich Kowalski, and Dieter Blaas

 

9. Capillary Electrophoresis, Gas Phase Electrophoretic Mobility Molecular Analysis, and Electron Microscopy - Effective Tools for Quality Assessment and Basic Rhinovirus Research

                Victor U. Weiss, Xavier Subirats, Mohit Kumar, Shushan Harutyunyan, Irene Gösler, Heinrich Kowalski, and Dieter Blaas

 

10. Proteases of Human Rhinovirus – Role in Infection

                Lora M. Jensen, Erin J. Walker, David A. Jans, and Reena Ghildyal

 

11. A Protocol to Express and Isolate HRV16 3C Protease for Use in In vitro Protease Assays

                Lora M. Jensen, Erin J. Walker, and Reena Ghildyal

 

12. Reverse Genetics System for Studying Human Rhinovirus Infections

                Wai-Ming Lee, Wensheng Wang, Yury A. Bochkov, and Iris Lee

 

13. Reverse Genetic Engineering of the Human Rhinovirus Serotype 16 Genome to Introduce an Antibody-Detectable Tag

                Erin J. Walker, Lora M. Jensen, and Reena Ghildyal

 

14. Mouse Models of Rhinovirus Infection and Airways Disease

                Nathan W. Bartlett, Aran Singanayagam, and Sebastian L. Johnston