Rheumatoid Arthritis: Methods and Protocols

ISBN-13: 9781493993932 / Angielski / Miękka / 2019 / 253 str.

Shuang Liu

Spis treści

Part I: Animal Models

1. Collagen-induced Arthritis Models

Miyoshi Maya and Shuang Liu

 

2. Human Xenograft Model

Shuang Liu

 

3. Long-term Constant Subcutaneous Drug Administration

Shuang Liu and Maya Miyoshi

 

4. Clinical Scoring of Disease Activity in Animal Models

Maya Miyoshi and Shuang Liu

 

5. Histological Analysis of Arthritic Joints

Takeshi Kiyoi

 

6. Preparation of Joint Extracts

Shuang Liu and Erika Takemasa

 

Part II: Therapeutic Approach

7. Production of Immunizing Antigen Proteoliposome Using Cell-free Protein Synthesis System

Wei Zhou and Hiroyuki Takeda

 

8. Re-construction of Protein/Liposome Complex

Yasuyuki Suzuki

 

9. Production of Neutralizing Antibody

Erika Takemasa, Shuang Liu, and Hitoshi Hasegawa

 

10. Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen

Hiroyuki Takeda

 

11. Generation of Specific Aptamers

Shuang Liu, Yasuyuki Suzuki, and Makoto Inui

 

12. Production of Lentiviral Particles

Shuang Liu

13. RNA Interference Ex Vivo

Shuang Liu

 

14. Lentiviral-mediated Systemic RNA Interference in vivo

Shuang Liu

 

15. Mesenchymal Stem Cell Engineering

Shuang Liu

 

Part III: Evaluation of Immunological Status

16. Screening of Ca²⁺ Influx in Lymphocytes

Erika Takemasa and Shuang Liu

 

17.       Single-cell Ca²⁺ Imaging

Shuang Liu

 

18. Electrophysiological Methods to Measure Ca²⁺ Current

Shuang Liu and Miyuki Kuno

 

19. The Functional Assessment of T cells

Saho Maruyama

 

20. Release of Antibodies and Cytokines from B cells

Shuang Liu

 

21. Evaluation of Autoreactive Responses

Shaung Liu

 

22. Bone-resorption Activity in Mature Osteoclasts

Takeshi Kyoi

 

23. Animal Models of Vasculitis

Mogi M and Shuang Liu

 

Part IV: Clinical Approach

24. Design an Intervention Study

Hitoshi Hasegawa

25. Assessment of Disease Activity, Structural Damage, and Function in Rheumatoid Arthritis

Hitoshi Hasegawa

Blurb

This volume provides a summary of the standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study.