Retinal Ganglion Cells

ISBN-13: 9781071634080 / Angielski / Twarda / 2023

Opis

This collection provides methods and techniques employed when culturing, manipulating, quantifying, and functionally assessing retinal ganglion cells (RGCs), vital for better understanding various ocular diseases and for developing novel therapies. The book features exhaustive and detailed protocols for the study of RGCs at both the in vitro and in vivo level, including the culture of rodent and human cells, and immunohistochemical, morphological, and functional assessments of various in vivo models of RGC death. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Retinal Ganglion Cells: Methods and Protocols serves as an ideal guide to these important cellular features of the central nervous system and the many traumatic and degenerative blinding diseases that affect them.

Spis treści

1. Isolation and Culture of Primary Retinal Ganglion Cells from Rodent Retina

            Esmahan Durmaz, Matyas Kutnyanszky, and Ben Mead

 

2. Purification of Retinal Ganglion Cells from Differentiation through Adult Via Immunopanning and Low-Pressure Flow Cytometry

            Sean M. Riordan, Afnan M. Aladdad, Kiran J. McLoughlin, and Karl E. Kador

 

3. Preparation of Retinal Explant Cultures

            Zubair Ahmed

 

4. Gene Gun DiOlistic Labeling of Retinal Ganglion Cells

            Gloria Cimaglia, Ryan J. Bevan, Andrew Want, and James E. Morgan

 

5. Differentiation of Human Embryonic/Induced-Pluripotent Stem Cells to Retinal Ganglion Cells

            Maryam Esmaeili and Ben Mead

 

6. Laser-Induced Ocular Hypertension in a Mouse Model of Glaucoma

            José M. Ramirez, Elena Salobrar-García, Rosa de Hoz, Juan J. Salazar, José A. Matamoros, Lidia Sánchez-Puebla, Inés López-Cuenca, José A. Fernández-Albarral, and Ana I. Ramírez

 

7. Silicone Oil-Induced Ocular Hypertension Glaucoma Model (SOHU) in Rodent and Non-Human Primate

            Fang Fang, Jie Zhang, and Yang Hu

 

8. Microbead-Induced Ocular Hypertension in a Rodent Model of Glaucoma

            Ben Mead

 

9. Viral Vector-Induced Ocular Hypertension in Mice

            Cameron Millar, Yogapriya Sundaresan, Gulab S. Zode, and Abbot F. Clark

 

10. Optic Nerve Crush Injury in Rodents to Study Retinal Ganglion Cell Neuroprotection and Regeneration

            Zubair Ahmed

 

11. Optical Coherence Tomography: Imaging Visual System Structures in Mice

            Xiangxiang Liu, Yuan Liu, and Richard K. Lee

 

12. Confocal Scanning Laser Ophthalmoscopy to Image Retinal Ganglion Cells in Real-Time

            Lili Hao, Yuan Liu, Xiangxiang Liu, and Richard K. Lee

 

13. In Vivo Detection of Retinal Ganglion Cell Stress in Rodents with DARC

            Daniel Hill, Soyoung Choi, and Maria Francesca Cordeiro

 

14. Measuring the Full-Field Electroretinogram in Rodents

            Pei Ying Lee, Da Zhao, Vickie H.Y. Wong, Anh Hoang, Katie K.N. Tran, Anna K. van Koeverden, Brianna C. Afiat, Christine T.O. Nguyen, and Bang V. Bui

 

15. Evaluation of Retinal Ganglion Cell Via Visual Evoked Potential

            Shengjian Lu, Tian Xia, and Yikui Zhang

 

16. High-Throughput Binocular Pattern Electroretinograms in the Mouse

            Tsung-Han Chou, Jonathon Toft-Nielsen, and Vittorio Porciatti

 

17. Intravitreal Injection of AAV for the Transduction of Mouse Retinal Ganglion Cells

            Bart Nieuwenhuis and Andrew Osborne

 

18. Methods to Identify Rat and Mouse Retinal Ganglion Cells in Retinal Whole Mounts

            Juan A. Miralles de Imperial-Ollero, Beatriz Vidal-Villegas, Alejandro Gallego-Ortega, Francisco M. Nadal-Nicolás, Manuel Salinas-Navarro, María Norte-Muñoz, Johnny Di Pierdomenico, Caridad Galindo-Romero, Marta Agudo-Barriuso, Manuel Vidal-Sanz, and Francisco J. Valiente-Soriano