Retinal Degeneration: Methods and Protocols

ISBN-13: 9781627030793 / Angielski / Twarda / 2012 / 384 str.

Bernhard H. F. Weber; Thomas Langmann

Opis

Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In Retinal Degeneration: Methods and Protocols, expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and techniques for addressing cell culture systems and animal models of disease, their generation, their phenotypic and molecular characterization as well as their use in therapeutic approaches to the retina. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Retinal Degeneration: Methods and Protocols aids scientists in continuing to study the cutting-edge techniques of retinal cell biology in health and disease.

Recenzje

From the book reviews:

"This step-by-step guide to studying retinal cell biology in health and disease covers a large spectrum of techniques and animal model species. ... Certainly, this book is for basic scientists. It is a great tool for those based in laboratories, and researchers at all levels can benefit from the basics to key tips on troubleshooting cutting-edge techniques and avoiding pitfalls. ... This book is great in simplifying new cutting-edge techniques in studying retinal degeneration." (Ayham Skaf, Doody's Book Reviews, May, 2013)

Spis treści

Part I: Human Genetics

1. Identification and Analysis of Inherited Retinal Disease Genes

Kornelia Neveling, Anneke I. den Hollander, Frans P.M. Cremers, and Rob W.J. Collin

Part II : Mouse Models

2. Mouse Models for Studies of Retinal Degeneration and Diseases

Bo Chang

3. Retinal Fundus Imaging in Mouse Models of Retinal Diseases

Anne F. Alex, Peter Heiduschka. Nicole Eter

4. Functional Phenotyping of Mouse Models with ERG

Naoyuki Tanimoto, Vithiyanjali Sothilingam, and Mathias W. Seeliger

5. Phenotyping of Mouse Models with OCT

M. Dominik Fischer, Ahmad Zhour and Christoph J. Kernstock

6. Light Damage as a Model of Retinal Degeneration

Christian Grimm and Charlotte E. Remé

7. N-Methyl-D-Aspartate (NMDA)-mediated Excitotoxic Damage – A Mouse Model of Acute Retinal Ganglion Cell Damage

Roswitha Seitz and Ernst R. Tamm

Part III: Other Animal Models

8. Generation of Transgenic X. laevis Models of Retinal Degeneration

Beatrice M. Tam, Christine C-L. Lai, Zusheng Zong and Orson L. Moritz

9. Analysis of Photoreceptor Degeneration in the Zebrafish Danio rerio

Holger Dill, Bastian Linder, Anja Hirmer and Utz Fischer

10. Analysis of Optokinetic Response in Zebrafish by Computer Based Eye Tracking

Sabina P. Huber-Reggi, Kaspar P. Mueller, Stephan C.F. Neuhauss

11. Analysis of the Drosophila Compound Eye with Light and Electron Microscopy

Monalisa Mishra and Elisabeth Knust

Part IV : In Situ Analyses

12. Cell Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy

Christiana L. Cheng, Hidayat Djajadi and Robert S. Molday

13. A Method of Horizontally-Sliced Preparation of the Retina

Ryosuke Enoki and Amane Koizumi

14. Detection of DNA Fragmentation in Retinal Apoptosis by TUNEL

Francesca Doonan and Thomas G. Cotter

15. High-throughput RNA in situ Hybridization in Mouse Retina

Seth Blackshaw

16. Assessment of Mitochondrial Damage in Retinal Cells and Tissues using Quantitative Polymerase Chain Reaction for Mitochondrial DNA Damage and Extracellular Flux Assay for Mitochondrial Respiration Activity

Stuart G. Jarrett, Bärbel Rohrer, Nathan R Perron, Craig Beeson, and Michael E. Boulton

17. Analysis of Photoreceptor Rod Outer Segment Phagocytosis by RPE Cells in situ

Saumil Sethna and Silvia C. Finnemann

Part V: Tissue Culture and Cell Models

18. Ca²⁺ Microfluorimetry in Retinal Müller Glial Cells

Antje Wurm, Thomas Pannicke, Andreas Reichenbach

19. Functional Analysis of Retinal Microglia and their Effects on Progenitors

Debra A Carter, Balini Balasubramaniam and Andrew D Dick

20. Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture

Yingyu Mao and Silvia C. Finnemann

21. Ca²⁺ Imaging Techniques to Analyze Ca²⁺ Signaling in Cells and to Monitor Neuronal Activity in the Retina

Olaf Strauß

Part VI: Gene Regulation

22. Double Chromatin Immunoprecipitation: Analysis of Target Co-Occupancy of Retinal Transcription Factors

Guang-Hua Peng and Shiming Chen

23. Quantifying the Activity of cis-regulatory Elements in the Mouse Retina by Explant Electroporation

Cynthia L. Montana, Connie A. Myers, and Joseph C. Corbo

Part VII: Therapy

24. Optimized Technique for Subretinal Injections in Mice

Regine Mühlfriedel, Stylianos Michalakis, Marina Garcia Garrido, Martin Biel, Mathias. W. Seeliger

25. Adeno Associated Viral Vectors for Gene Therapy of Inherited Retinal Degenerations

John G. Flannery and Meike Visel

26. Barrier Modulation in Drug Delivery to the Retina

Matthew Campbell, Marian Humphries and Peter Humphries