Retinal Degeneration: Methods and Protocols

ISBN-13: 9781493993604 / Angielski / Miękka / 2019 / 417 str.

Bernhard H. F. Weber; Thomas Langmann

Spis treści

Part I: Molecular Genetics and Tools

 

1      Identification and Analysis of Genes Associated with Inherited Retinal Diseases

Mubeen Khan, Zeinab Fadaie, Stéphanie S. Cornelis, Frans P.M. Cremers, and Susanne Roosing

 

2      eQTL Analysis in Retinal Research

Felix Graßmann

 

3      Testing for known Retinal Degeneration Mutants in Mouse Strains

Khalid Rashid, Katharina Dannhausen, and Thomas Langmann

 

4      CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo

Daniela Benati, Valeria Marigo, and Alessandra Recchia

 

5    Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis

Yuchen Lin, Sarah Irmscher, and Christine Skerka

 

Part II: Cell/Tissue Culture Models

 

6       Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells

Caroline Brandl

 

7       Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture

Francesca Mazzoni, Yingyu Mao, and Silvia C. Finnemann

 

8       Porcine RPE/Choroidal Explant Cultures

Alexa Klettner and Yoko Miura

 

9      The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Embryonic Stem Cells

Manuela Völkner, Thomas Kurth, and Mike O. Karl

 

10    Cell Death Analysis in Retinal Cultures

Sarah L Roche, Ana M Ruiz-Lopez, and Thomas G Cotter

 

11    Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-derived Macrophages in Retinal Disease

Nancy J Reyes, Rose Mathew, and Daniel R Saban

 

Part III: Animal Models

 

12    Light Damage Models of Retinal Degeneration

Christian Grimm and Charlotte E. Reme

 

13    Induction and Readout of Oxygen-Iinduced Retinopathy

Raffael Liegl, Claudia Priglinger, and Andreas Ohlmann

 

14    Generation and Analysis of Xenopus laevis Models of Retinal Degeneration using CRISPR/Cas9

Joanna M. Feehan, Paloma Stanar, Beatrice M. Tam, Colette Chiu, and Orson L. Moritz

 

15    Gene Knockdown in Zebrafish (Danio rerio) as a Tool to Model Photoreceptor Diseases

Holger Dill and Utz Fischer

 

16    Drosophila melanogaster - A Valuable Genetic Model Organism to Elucidate the Biology of Retinitis Pigmentosa

Malte Lehmann, Elisabeth Knust, and Sarita Hebbar

 

Part IV: Retinal Imaging

 

17    Retinal Fundus Imaging in Mouse Models of Retinal Diseases

Anne F. Alex, Maged Alnawaiseh, Peter Heiduschka, and Nicole Eter

 

18    Phenotyping of Mouse Models with OCT

G. Alex Ochakovski and M. Dominik Fischer

 

19    Cell-specific Markers for the Identification of Retinal Cells and Subcellular Organelles by Immunofluorescence Microscopy

Laurie L. Molday, Christiana L. Cheng, and Robert S. Molday

 

20    Immuno-TEM/STEM in Retinal Research

Sanae Sakami and Krzysztof Palczewski

 

21    Noninvasive Two-photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium

Grazyna Palczewska, Timothy S. Kern, and Krzysztof Palczewski

 

Part V: Therapies

 

22    Cell-based Therapy for Retinal Disease: The New Frontier

Marco Zarbin

 

23    In Vitro Evaluation of AAV Vectors for Retinal Gene Therapy

Johanna Wagner, Christian Schön, Elvir Becirovic, Martin Biel, and Stylianos Michalakis

 

24    Nanoparticles Targeting Retinal and Choroidal Capillaries In Vivo

Alexandra Haunberger and Achim Goepferich

 

25    Optimized Subretinal Injection Technique for Gene Therapy Approaches

Regine Mühlfriedel, Stylianos Michalakis, Marina Garcia Garrido, Vithiyanjali Sothilingam, Christian Schön, Martin Biel, and Mathias W. Seeliger

Blurb

This volume provides key updates on several of the first edition chapters and also includes new novel techniques, addressing the most recent technological developments and their applications in retinal research. Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Retinal Degeneration: Methods and Protocols, Second Edition aims to ensure successful scientific work in the further study of this vital field.