Reporter Gene Assays: Methods and Protocols

ISBN-13: 9781493992683 / Angielski / Miękka / 2019 / 234 str.

Robert Damoiseaux; Samuel Hasson

Spis treści

Preface…

Foreword…

Table of Contents…

Contributing Authors…

1. Genome-Edited Cell Lines for High-Throughput Screening

Patricia Dranchak, John Moran, Ryan MacArthur, Camila Lopez-Anido, James Inglese, and John Svaren

2. High-Throughput Firefly Luciferase Reporter Assays

Ellen Siebring-van Olst and Victor W. van Beusechem

3. Using the 2A Protein Co-Expression System: Multi-Cistronic 2A Vectors Expressing Gene(s) of Interest and Reporter Proteins

Garry A. Luke and Martin D. Ryan

4. Developing Mammalian Cellular Clock Models Using Firefly Luciferase Reporter

Chidambaram Ramanathan and Andrew Liu

5. High-Throughput Screening Method to Identify Alternative Splicing Regulators

Cheryl Stork and Sika Zheng

6. High-Throughput Screening of a Luciferase Reporter of Gene Silencing on the Inactive X Chromosome

Alissa Minkovsky, Kathrin Plath, and Robert Damoiseaux

7. Making It All Work: Functional Genomics and Reporter Gene Assays

Genevieve Welch, Robert Damoiseaux, and Loren Miraglia

8. Reporter Gene Assays Using Transfectable Functional Genomics Libraries

Genevieve Welch, Robert Damoiseaux, and Loren Miraglia

9. Reporter Gene Assays Using Viral Functional Genomics Libraries

Genevieve Welch, Robert Damoiseaux, and Loren Miraglia

10. Using YFP as a Reporter of Gene Expression in the Green Alga Chlamysomonas reinhardtii

Crysten E. Blaby-Haas, M. Dudley Page, and Sabeeha S. Merchant

11. Gene Expression Analysis by Arylsulfatase Assays in the Green Algo Chlamydomonas reinhardtii

Ian K. Blaby and Crystem E. Blaby-Haas

12. Endogenous Locus Reporter Assays

Yaping Liu, Jeffrey Hermes, Jing Li, and Matthew Tudor

13. High Content Reporter Assays

Erica Cook, Jeffrey Hermes, Jing Li, and Matthew Tudor

14. Data Mining and Computational Modeling of High-Throughput Screening Datasets

Sean Ekins, Alex M. Clark, Krishna Dole, Kellan Gregory, Andrew M. McNutt, Anna Coulon Spektor, Charlie Weatherall, Nadia K. Litterman, and Barry A. Bunin

15. Intravital Imaging by Two-Photon Excitation Microscopy of Human Melanoma Cells in the Mouse Ear Skin

Nathan Y. Z. Bentolila, Raymond L. Barnhill, Claire Lugassy, and Laurent A. Bentolila

Blurb

This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs.  Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interested in learning more about reporter genes.