Regulatory B Cells: Methods and Protocols

ISBN-13: 9781071612361 / Angielski / Twarda / 2021 / 471 str.

Francesca Mion; Silvia Tonon

Spis treści

Part I: Purification and Phenotypic Characterization of B Cell Subsets with Breg Traits

 

1.                  Purification and characterization of murine MZ and T2-MZP cells

M. Manuela Rosado, Alaitz Aranburu, Marco Scarsella, Simona Cascioli, Ezio Giorda, and Rita Carsetti

2.                  Purification and immune-phenotyping of B-1 cells from body cavities of mice Vanessa Yenson and Nicole Baumgarth

3.                  Purification and immunophenotypic characterization of murine plasma cells Van Duc Dang, Simon Fillatreau and Andreia C. Lino

4.                  Purification of murine and human IL-10 producing B cells from different anatomical compartments

Francesca Mion, Eleonora Martinis, Carlo E.M. Pucillo and Silvia Tonon

5.                  Purification and immunophenotypic characterization of human CD19⁺CD24^hiCD38^hi and CD19⁺CD24^hiCD27⁺ B cells

Hannah F. Bradford and Claudia Mauri

  Part II: Mechanisms of Immune Suppression by B Cells

 

6.                  Detection of IL-10 in murine B cells: in vitro and in vivo techniques

Xiang Lin, Xiaohui Wang and Liwei Lu

7.                  Detection and quantification of transforming growth factor-β1 produced by murine B cells: pros and cons of different techniques

Yoshiyuki Mishima, Akihiko Oka and Shunji Ishihara

8.                  IL-35 detection in B cells at the mRNA and protein level

Bhalchandra Mirlekar, Daniel Michaud and Yuliya Pylayeva-Gupta

9.                  Characterization and activation of Fas Ligand producing mouse B cells and their killer exosomes

Steven K. Lundy, Sophina H. Taitano, and Luciën E.P.M. van der Vlugt

10.              Characterization and activity of TIM-1 and IL-10-reporter expressing regulatory B cells

Kanishka Mohib, David M. Rothstein and Qing Ding

11.              New method for the expansion of highly purified human regulatory granzyme B-expressing B cells

Mélanie Chesneau, Hoa L. Mai and Sophie Brouard

12.              Characterization of the cell surface phenotype and regulatory activity of B cell IgD low (BD_L) regulatory B cells

Mohamed Khalil, Avijit Ray and Bonnie N. Dittel

Part III: Methods for the ex vivo expansion and molecular characterization of IL-10 producing B cells

 

13.              Use of Toll-like receptor (TLR) ligation to characterize human regulatory B cells subsets

Mathilde A.M. Chayé, Chiara Tontini, Arifa Ozir-Fazalalikhan, Astrid L. Voskamp and Hermelijn H. Smits

14.              Use of co-cultures for the study of cellular interactions influencing B cell regulatory functions

Giada Pace and Viviana Valeri

15.              Use of inhibitory compounds to dissect the molecular pathways involved in regulatory B cell differentiation

Maik Luu and Alexander Visekruna

16.              Methods to study the transcriptome of regulatory B cells

Maud Maho-Vaillant and Sebastien Calbo

17.              Purification of murine IL-10⁺ B cells for analyses of biological functions and transcriptomics

Jianbo Sun and Uttiya Basu

18.              il-10 gene locus DNA methylation in regulatory B cells

Silvia Tonon, Eleonora Martinis, Carlo E.M. Pucillo and Francesca Mion

19.              B cell commitment to IL-10 production: the Vert-X Il10^egfp mouse

Akihiko Oka, Bo Liu, Jeremy W. Herzog and R. Balfour Sartor

Part V: Study of regulatory B cells in pathological settings 

20.              Regulatory B cells in experimental mouse models of arthritis

Diana E. Matei, Claudia Mauri and Elizabeth C. Rosser

21.              Regulatory and IgE⁺ B cells in allergic asthma

John Paul Oliveria, Rita Agayby and Gail M. Gauvreau

22.              Regulatory B cells in type 1 diabetes

Joanne Boldison, Larissa Camargo Da Rosa and F. Susan Wong

23.              Analysis of regulatory B cells in experimental autoimmune uveitis

Jin Kyeong Choi and Charles E. Egwuagu

24.              Purification and immunophenotypic characterization of human CD24^hiCD38^hi and CD24^hiCD27⁺ regulatory B cells in transplantation

Rowa. Y. Alhabbab and Giovanna Lombardi

Blurb

 This second edition provides new and updated chapters useful for the study of Regulatory B cells. Organized in four sections, chapters detail basic methods for the isolation and immunophenotypical analysis of these cells, experimental approaches for the ex vivo generation/expansion of IL-10 producing B cells, molecular biology techniques for the analysis of IL-10 expression and production, and animal models mimicking pathologic settings. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Regulatory B Cells: Methods and Protocols aims to be useful to the scientific community and serve to clarify some unsolved aspects of Regulatory B Cells research.