Recombineering: Methods and Protocols

ISBN-13: 9781071622322 / Angielski / Twarda / 2022

Reisch, Christopher R.

Opis

This volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Recombineering: Methods and Protocols is a valuable resource for any researchers interested in learning more about this developing field. 

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. Scarless ssDNA Recombineering of Phage in the Lysogenic State
Maria Ababi, Matthew Tridgett, Alexander Osgerby, and Alfonso Jaramillo

2. Lambda red Recombineering of Bacteriophage in the Lysogenic State 
Matthew Tridgett, Maria Ababi, and Alfonso Jaramillo

3. Recombineering Mediated Genome Editing in Burkholderiales Strains
Xue Wang, Jiaqi Liu, Wentao Zheng, Youming Zhang, and Xiaoying Bian

4. High-Efficiency Multi-Site Genomic Editing (HEMSE) Made Easy
Tomás Aparicio, Víctor de Lorenzo, and Esteban Martínez-García

5. Creation of Markerless Genome Modifications in a Non-Model Bacterium by Fluorescence-Aided Recombineering
Piyush Behari Lal, Fritz Wells, and Patricia J. Kiley

6. Plant Gene Modification by BAC Recombineering
Zhoubo Hu, Ajit Ghosh, and Csaba Koncz

7. Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling
Joakim Näsvall

8. Genome Editing in Klebsiella pneumoniae using CRISPR/Cas9 Technology
Zhipeng Wang, Yu Wang, and Quanjiang Ji

9. Generating Single Nucleotide Point Mutations in E. coli with the No-SCAR System
Adam J. Ellington and Christopher R. Reisch

10. Recombineering in Staphylococcus aureus
Kelsi Penewit and Stephen J. Salipante

11. Genome Editing Methods for Bacillus subtilis
Katherine J. Wozniak and Lyle A. Simmons

12. Highly Efficient Genome Editing in Clostridium difficile using the CRISPR-Cpf1 System
Wei Hong, Jie Zhang, Guzhen Cui, Qian Zhou, Pixiang Wang, and Yi Wang

13. Genome Editing of Corynebacterium glutamicum using CRISPR-Cpf1 System
Zhiqiang Wen, Fenghui Qian, Jiao Zhang, Yu Jiang, and Sheng Yang

14. CRISPR/Cas-Mediated Genome Editing of Streptomyces
Lee Ling Tana, Elena Heng, Nadiah Zulkarnain, Wan-Chi Hsiao, Fong T. Wonga, and Mingzi M. Zhang

15. Cas9 Nickase-Based Genome Editing in Clostridium cellulolyticum
Tao Xu, Xuanyu Tao, Megan L. Kempher, and Jizhong Zhou

16. Genome Editing with Cas9 in Lactobacilli
Justin M. Vento and Chase L. Beisel

Subject Index List…