Recombinant Gene Expression

ISBN-13: 9781493962211 / Angielski / Miękka / 2016 / 649 str.

Argelia Lorence

Opis

Recombinant gene expression research has brought new advances such as the birth of the 'omics' technologies. Here, expert researchers detail many RGE methods now used, including those for bacteria, fungi, plants and plant cells, and animals and animal cells.

Spis treści

Table of Contents

 “Recombinant Gene Expression: Reviews and Protocols, Third Edition”

Argelia Lorence (Editor)

 

Name of corresponding author(s) is underlined

 

General Aspects

1)     Using Folding Promoting Agents in Recombinant Protein Production: A Review

 Beatrix Fahnert

2)    Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass Spectrometry

Brett J. Savary and Prasanna Vasu

 

3)    A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene Expression

María de la Cruz Muñoz-Centeno, Gonzalo Millán-Zambrano and Sebastián Chavez

 

4)    Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant Proteins

Maureen C. Dolan, Giuliana Medrano, Anthony McMickle, and Carole L. Cramer

 

Prokaryotes

5)    Heat-shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli

 Christos A. Kyratsous and Christos A. Panagiotidis

 

6)    The use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in Salmonella

Ferenc Vonderviszt, Rachel Sajo, Jozsef Dobo, Peter Zavodoszky

 

7)    Optimization of Purification Protocols Based on the Step-by-step Monitoring of the Protein Aggregates in the Soluble Fractions

Ario De Marco

 

8)            Heterologous Protein Expression by Lactococcus lactis

Julio Villatoro-Hernández, Oscar Paul Kuipers, Odila Saucedo-Cárdenas and Roberto Montes de Oca Luna

 

9)    An Extended Suite of Genetic Tools for Use in Bacteria of the Halomonadaceae: An Overview

 Monserrat  Argandoña, Carmen Vargas, Javier Rodríguez-Moya, Mercedes Reina-Bueno, Manuel Salvador and Joaquin J. Nieto

 

10)  Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125

Valentina Rippa, Rosanna Papa, Maria Giuliani, Cinzia Pezzella, Ermenegilda Parrilli, Maria Luisa Tutino, Gennaro Marino, and Angela Duilio

 

11) A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125

Maria Giuliani, Ermenegilda Parrilli , Cinzia Pezzella,Valentina Rippa, Angela Duilio, Gennaro Marino and Maria Luisa Tutino

 

12) A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-protein Interaction

Jumei Zeng and Zheng-Guo He

 

13) Heterologous High-level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatus

Nadine Katzke, René Bergmann, Karl-Erich Jaeger Thomas Drepper

 

14) Plasmid DNA Production for Therapeutic Applications

Alvaro Lara and Octavio T. Ramirez

 

Lower eukaryotes

15) Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review

Tomoaki Niimi

 

16) Expression of Multi-subunit Proteins in Leishmania tarentolae

Marisa Sugino and Tomoaki Niimi

 

Fungi

17) Recombinant Protein Production in Yeasts

Diethard Mattanovich, Paola Branduardi, Laura Dato, Brigitte Gasser, Michael Sauer and Danilo Porro

 

18) Yeasts as Tool for Heterologous Gene Expression

Raja Mokdad-Gargouri,  Salma Abdelmoula-Soussi, Nadia Hadiji-Abbès, Ines Yacoubi-Hadj Amor, Istabrak Borchani-Chabchoub and Ali Gargouri

 

19) The Cre/lox system: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal Endophytes

Simona Florea, Caroline Machado, Kalina Andreeva, and Christopher L. Schardl

20)  Aptamer-regulated Expression of Essential Genes in Yeast

Beatrix Suess, Karl-Dieter Entian, Peter Kötter and Julia E. Weigand

 

21) Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris

Prasanna Vasu, Stefan Bauer, and Brett J. Savary 

 

22) A Thiamine-regulatable Epitope-tagged Protein Expression System in Fission Yeast Tiina Tamm

 

23) Heterologous Gene Expression by Chromosomal Integration in Fission Yeast

Akihisa Matsuyama and Minoru Yoshida

 

24) Genetic Engineering of Industrial Strains of Saccharomyces cerevisiae

Sylvie Le Borgne Le Gall

 

Plants and Plant Cells

25) Recombinant Protein Production in Plants: Challenges and Solutions

Elizabeth E. Hood and Deborah V. Vicuña Requesens

 

26) A Novel Plant Cell Bioproduction Platform for High-yield Secretion of Recombinant Proteins

Jianfeng Xu and Marcia J. Kieliszewski

27) Super-promoter:TEV, a Powerful Gene Expression System for Tobacco Hairy Roots

Luis Ñopo, Bonnie J. Woffenden, Deborah Reed, Scott Buswell, Chenming Zhang,  and Fabricio Medina-Bolivar

 

28) Bioseparation of Recombinant Proteins from Plant Extract with Hydrophobin Fusion Technology

Jussi J Joensuu, Andrew J. Conley, Markus B. Linder, Rima Menassa

 

29) Quality Assessment of Recombinant Proteins Produced in Plants  

Giuliana Medrano, Maureen C. Dolan, Jose Condori, David  N. Radin, and Carole L. Cramer

 

30) Cell-free Protein Synthesis as a Promising Expression System for Recombinant Proteins

 Xumeng Ge and Jianfeng Xu

Animals and Animal Cells

31) The use of Bacterial Artificial Chromosomes for Recombinant Protein Production in Mammalian Cell Lines

Leander Blaas, Monica Musteanu, Beatrice Grabner, Robert Eferl, Anton Bauer, and Emilio Casanova

 

32) Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and Authenticity

Lyne Jossé, Mark Smales, and Mick F. Tuite

 

33) High-throughput Baculovirus Expression in Insect Cells Richard B. Hitchman Robert D. Possee, and Linda A. King

 

34) Recombinant Protein Expression in Milk of Livestock Species

Zsuzsanna Bősze and Laszló Hiripi

 

 

11) A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125

Maria Giuliani, Ermenegilda Parrilli , Cinzia Pezzella,Valentina Rippa, Angela Duilio, Gennaro Marino and Maria Luisa Tutino

 

12) A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-protein Interaction

Jumei Zeng and Zheng-Guo He

 

13) Heterologous High-level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatus

Nadine Katzke, René Bergmann, Karl-Erich Jaeger Thomas Drepper

 

14) Plasmid DNA Production for Therapeutic Applications

Alvaro Lara and Octavio T. Ramirez

 

Lower eukaryotes

15) Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review

Tomoaki Niimi

 

16) Expression of Multi-subunit Proteins in Leishmania tarentolae

Marisa Sugino and Tomoaki Niimi

 

Fungi

17) Recombinant Protein Production in Yeasts

Diethard Mattanovich, Paola Branduardi, Laura Dato, Brigitte Gasser, Michael Sauer and Danilo Porro

 

18) Yeasts as Tool for Heterologous Gene Expression

Raja Mokdad-Gargouri,  Salma Abdelmoula-Soussi, Nadia Hadiji-Abbès, Ines Yacoubi-Hadj Amor, Istabrak Borchani-Chabchoub and Ali Gargouri

 

19) The Cre/lox system: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal Endophytes

Simona Florea, Caroline Machado, Kalina Andreeva, and Christopher L. Schardl

20)  Aptamer-regulated Expression of Essential Genes in Yeast

Beatrix Suess, Karl-Dieter Entian, Peter Kötter and Julia E. Weigand

 

21) Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris

Prasanna Vasu, Stefan Bauer, and Brett J. Savary 

 

22) A Thiamine-regulatable Epitope-tagged Protein Expression System in Fission Yeast Tiina Tamm

 

23) Heterologous Gene Expression by Chromosomal Integration in Fission Yeast

Akihisa Matsuyama and Minoru Yoshida

 

24) Genetic Engineering of Industrial Strains of Saccharomyces cerevisiae

Sylvie Le Borgne Le Gall

 

Plants and Plant Cells

25) Recombinant Protein Production in Plants: Challenges and Solutions

Elizabeth E. Hood and Deborah V. Vicuña Requesens

 

26) A Novel Plant Cell Bioproduction Platform for High-yield Secretion of Recombinant Proteins

Jianfeng Xu and Marcia J. Kieliszewski

27) Super-promoter:TEV, a Powerful Gene Expression System for Tobacco Hairy Roots

Luis Ñopo, Bonnie J. Woffenden, Deborah Reed, Scott Buswell, Chenming Zhang,  and Fabricio Medina-Bolivar

 

28) Bioseparation of Recombinant Proteins from Plant Extract with Hydrophobin Fusion Technology

Jussi J Joensuu, Andrew J. Conley, Markus B. Linder, Rima Menassa

 

29) Quality Assessment of Recombinant Proteins Produced in Plants  

Giuliana Medrano, Maureen C. Dolan, Jose Condori, David  N. Radin, and Carole L. Cramer

 

30) Cell-free Protein Synthesis as a Promising Expression System for Recombinant Proteins

 Xumeng Ge and Jianfeng Xu

Animals and Animal Cells

31) The use of Bacterial Artificial Chromosomes for Recombinant Protein Production in Mammalian Cell Lines

Leander Blaas, Monica Musteanu, Beatrice Grabner, Robert Eferl, Anton Bauer, and Emilio Casanova

 

32) Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and Authenticity

Lyne Jossé, Mark Smales, and Mick F. Tuite

 

33) High-throughput Baculovirus Expression in Insect Cells Richard B. Hitchman Robert D. Possee, and Linda A. King

 

34) Recombinant Protein Expression in Milk of Livestock Species

Zsuzsanna Bősze and Laszló Hiripi

 

Blurb

Studies related to recombinant gene expression have brought new advance such as  the emergence of the “omics” technologies. While Escherichia coli, Sacharomyces cerevisiae and insect cells continue to be the dominant production platforms of recombinant proteins. In Recombinant Gene Expression: Review and Protocols, Third Edition, expert researchers in the field detail many of the methods now commonly used to study recombinant gene expression. These include methods and techniques for bacteria, lower eukaryotes, fungi, plants and plant cells, and animals and animal cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Recombinant Gene Expression: Review and Protocols, Third Edition seeks to aid scientists in the further study of this crucially important research into recombinant gene expression.