Recombinant Enzymes - From Basic Science to Commercialization

ISBN-13: 9783319356051 / Angielski / Miękka / 2016 / 188 str.

Azura Amid

Spis treści

1. Introduction to Recombinant Enzyme Pre-commercialization; Azura Amid

2. Recombinant Enzyme: Cloning and Expression; Azura Amid and Norhidayah Hassan

3. Common Laboratory Procedure; Azura Amid, Mohd Jamil Aizat Jamaluddin and Muhd Ezza Faize Othman

4. Characterization of Recombinant Enzymes; Farah Fadwa Ben Belgasem and Hamzah Mohd. Salleh 

5. Purification of Recombinant Protein for Industrial Use; Faridah Yusof

6. Recombinant Enzyme Fermentation; Azura Amid, Nurul Azira Ismail and Mohd Jamil Aizat Jamaluddin

7. Scaling-up Recombinant Enzyme Fermentation; Azlin Suhaida Azmi, Sarina Sulaiman, Nor Fadhillah Mohamed Amin, Fathilah Ali

8. Downstream Processing of Recombinant Enzymes for Commercialization; Fadzilah Adibah Abdul Majid 

9. Economic and Environmental Evaluation of Recombinant Enzyme Production; Mohd Jamil Aizat Jamaluddin, Azlin Suhaida Azmi, Sarina Sulaiman, Dzun Noraini Jimat, Muhd. Ezza Faiez Othman and Azura Amid

10. Case Study: Recombinant Bromelain Selection; Azura Amid, Nurul Azira Ismail and Zatul Iffah Mohd Arshad

11. Case Study: Recombinant Bromelain Cloning, Characterization and Upstream Processes; Azura Amid, Nurul Azira Ismail and Mohd Jamil Aizat Jamaluddin

12. Case Study: Recombinant Bromelain Downstream Processing; Azura Amid, Zatul Iffaf Mohd Arshad and Muhd Ezza Faiez Othman

 

Blurb

This edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product. 

Readers will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host. 

A discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production. 

This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization.