Receptor-Mediated Uptake in the Liver

ISBN-13: 9783540161813 / Angielski / Miękka / 1986 / 223 str.

H. Greten; E. Windler; U. Beisiegel

Opis

Cell surface receptors are multifunctional proteins with binding sites towards the external environment and effector sites which mediate intracellular events. The purpose of this symposium was to bring together investigators who have a com mon interest in those receptors which are located in the liver, and who have studied endocytic mechanisms for various macromolecules like insulin, lipoproteins, epi dermal growth factor and others. Experiments in this particular field of research date back to the early 60-ies but have only recently led to new and important in sight in the molecular basis of receptor mediated uptake in the liver. The structural features which control these mechanisms are currently under intense investigation in many laboratories. Though this symposium largely emphasizes lipoprotein up take and catabolism by the liver, it was the particular intention of the organizers to discuss methodology and results with investigators who are also interested in he patic uptake of macromolecules. This might then eventually lead to new and com mon concepts for both receptor-ligand interaction and internalization processes in the liver. Biochemists, pathologists and gastroenterologists met for two and a half days and discussed their latest data in this so rapidly developing field of basic rese arch. This conference is part of a series on current topics in gastroenterology and hepatology arranged regularly by the Departments of Medicine and Surgery at the University Hospital Eppendorf. If is our hope, that such exchange of information between the different disciplines in medicine will continue. i. k."

Spis treści

Mechanisms of Uptake and Processing of Macromolecules by the Liver.- The LDL Receptor — Structural Insights.- Partial Purification and Characterization of Hepatic Low Density Lipoprotein (LDL) Receptor from Rabbit Liver.- DNA Polymorphisms of the Low Density Lipoprotein (LDL) Receptor Gene.- LDL-Binding to Human Liver Plasma Membranes.- Detection of LDL Receptors in Liver Membranes by Ligand Blotting with Biotin-Modified Plasma Lipoproteins.- Cellular Localization of Lipoprotein Receptors in Liver.- Cell Density Dependent Uptake of LDL in Cultured Rat Hepatocytes.- APO-B, E Receptor Binding of LDL: Evidence for Species-Specific Segments of APO-B100 in the Binding Site Domain.- Effect of the Phorbol Esters on the Receptors for LDL on U-937 Monocyte-Like Cells and Acetyl-LDL on Mouse Peritoneal Macrophages.- Regulation of Low Density Lipoprotein Levels in Animals and Man with Particular Emphasis on the Role of the Liver.- Turnover of Light and Heavy Fractions of Human LDL in Normal and WHHL Rabbits.- Regulation of LDL Receptor Activity in Human Hepatocytes.- Non-Genetic Regulation of LDL Metabolism: Role of Lipid Transfer Proteins and Hypertriglyceridemia.- The Control of Cholesterol Homeostasis: Regulation of HMG CoA Reductase.- Cholesterol and Bile Acid Synthesis as Related to Receptor and Non-receptor Mediated Degradation of LDL in Man.- The Reticuloendothelial System and Low Density Lipoprotein Metabolism.- Removal of Chylomicron Remnants by the Hepatic LDL Receptor Liver — Possible Contribution of the Low Density Lipoprotein Receptor.- Metabolism of Human Triglyceride-rich Lipoprotein Remnants.- Suppression of Apoprotein-E Receptor by LP-X.- A Novel Mechanism by which High Density Lipoprotein Selectively Delivers Cholesterol Esters to the Liver.- Saturable Binding of HDL3 to Human Liver Membranes.- Subcellular Dissection and Characterization of Plasma Lipoprotein Secretory (Golgi) and Endocytic (Multivesicular Bodies) Compartments of Rat Hepatocytes.- Immunoelectron Microscopy on Receptor and Ligand Sorting Sites.- The Epidermal Growth Factor Receptor; Studies in Cultured Hepatocytes and During Liver Regeneration.- Insulin Interaction with Target Cells Morpho-Functional Aspects.- The Regulation and Dynamics of the Transferrin Receptor.- Receptor-Mediated Endocytosis of Mannose-Terminated Glycoproteins in Hepatocytes.- Rapid Clearance of Lipoproteins and Liposomes by Hepatic Parenchymal or Kupffer Cells by Incorporation of a Tris-Galactoside-Terminated Cholesterol Derivative.- Intracellular Degradation of Asialoglycoproteins in Rat Hepatocytes Studied by Fractionation in Nycodenz Gradients.- The Effect of Monensin on Receptor-Mediated Endocytosis of Asialofetuin and Secretion of Very Low Density Lipoproteins (VLDL) by Cultured Rat Hepatocytes.- Membrane Transport of Amphiphilic Compounds by Hepatocytes.- Studies on Hepatocellular Uptake of Fatty Acids.- Inter- and Intracellular Transport of Retinol in the Liver.- The Dynamics of Clathin and the Coated Vesicle Pathway.