Radiation Cytogenetics: Methods and Protocols

ISBN-13: 9781493994304 / Angielski / Twarda / 2019 / 138 str.

Takamitsu A. Kato; Paul F. Wilson

Spis treści

1. Human Lymphocyte Metaphase Chromosome Preparation for Radiation-Induced Chromosome Aberration Analysis

            Takamitsu A. Kato

 

2. Animal Lymphocyte Metaphase Chromosome Preparation

            Kazuki Heishima, Kendon Kuo, Masashi Kimura, and Takashi Mori

 

3. Micronuclei Formation Analysis after Ionizing Radiation

            Cathy Su, Alexis H. Haskins, and Takamitsu A. Kato

 

4. G1 Premature Chromosome Condensation (PCC) Assay

            Ryuichi Okayasu and Cuihua Liu

 

5. G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity

            Jeremy S. Haskins and Takamitsu A. Kato

 

6. G2 Premature Chromosome Condensation/Chromosome Aberrations Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology

            Eisuke Gotoh

 

7. Sister Chromatid Exchange as a Genotoxic Stress Marker

            Shigeaki Sunada, Jeremy S. Haskins, and Takamitsu A. Kato

 

8. DNA Damage Focus Formation Assay

            Yoshihiro Fujii

 

9. Nuclear Foci Assays in Live Cells

            Eiichiro Mori and Aroumougame Asaithamby

 

10. In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes

            Chisato Omata and Takamitsu A. Kato

 

11. PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations

            Ian M. Cartwright, Jeremy S. Haskins, and Takamitsu A. Kato

 

12. Modified PNA Telomere and Centromere FISH Protocols

            Ian M. Cartwright

 

13. Directional Genomic Hybridization (DGH) for Detection of Intra-Chromosomal Rearrangements

            Erin Robinson, Miles J. McKenna, Joel S. Bedford, Edwin H. Goodwin, Michael N. Cornforth, Susan M. Bailey, and F. Andrew Ray

 

14. Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH

            Jeremy S. Haskins and Takamitsu A. Kato

 

15. Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting

            Bradford D. Loucas

Blurb

This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.