RNAi Strategies for Pest Management: Methods and Protocols

ISBN-13: 9781071616352 / Angielski / Miękka / 2022

Vaschetto, Luis María

Opis

This volume explores the latest techniques used for sustainable development of eco-friendly pest management systems. Chapters in this book look at the design of novel RNAi-mediated pest control strategies and related issues. Some of the topics covered in this book are identification and characterization of differentially expressed non-coding RNAs in insect genomes; functional small RNA design; analysis of endogenous RNAi pathways; RNAi screening; plant models of RNAi-mediated pest control; and dsRNA delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, RNAi Strategies for Pest Management: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field. 

Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spis treści

Dedication…
Preface…
Table of Contents…
Contributing Authors…

1. Genome-Wide Identification and Functional Characterization of Non-Coding RNAs (ncRNAs) Differentially Expressed during Insect Development
Yue Wang, Minsheng You, and Weiyi He

2. Identification and Functional Characterization of Argonaute (Ago) Proteins in Insect Genomes
Bernard Moussian and Nicolas Casadei

3. Functional Characterization of Silkworm PIWI Proteins by Embryonic RNAi
Takashi Kiuchi and Susumu Katsuma

4. Guiding RNAi Design through Characterization of Endogenous Small RNA Pathways
Jacob O. Peter, Yulica Santos-Ortega, Alex Flynt

5. Silencing of Molecular Targets with Relevance to Insecticide Resistance in Colorado Potato Beetle using dsRNA
Raed Bouafoura, Mariem Ben Youssef, and Pier Jr Morin

6. Visualization of RNA Transcripts in Western Corn Rootworm (Diabrotica virgifera virgifera) and Plants by In Situ Hybridization
Joseph P. Steimel and Xu Hu

7. In Silico Screening of Attractive Target Genes for RNAi-Based Pest Control
Weilin Zhang

8. RNAi Feeding Bioassay: A Protocol for dsRNA Screening against Asian Citrus Psyllid and Related Hemipteran Insects
Jonatha dos Santos Silva, Layanna Rebouças de Santana Cerqueira, Wayne Bextine Hunter, Eduardo Chumbinho de Andrade

9. A Random-Screening Approach to Identify RNAi Targets for the Control of Western Corn Rootworm (Diabrotica. virgifera virgifera Le Conte)
Xu Hu and Adane Kassa

10. Rapid Screening of Myzus persicae (Green Peach Aphid) RNAi Targets using Tobacco rattle virus
Honglin Feng and Georg Jander

11. Genome-Wide Identification and Validation of Target Genes Associated with Insecticide Treatment of the Green Peach Aphid, Myzus persicae
Sadia Iqbal, John Fosu-Nyarko, Frances Brigg, and Michael G.K. Jones

12. Inhibition of Rhopalosiphum maidis (Corn Leaf Aphid) Growth on Maize by Virus-Induced Gene Silencing with Sugarcane mosaic virus
Seung Ho Chung and Georg Jander

13. RT-qPCR for Detection and Expression Monitoring of Core Genes Involved in the RNA Interference Pathways of Western Corn Rootworm (Diabrotica virgifera virgifera)
Courtney A. Davis-Vogel

14. Examination of the Suitability of Attractive Target Genes for RNAi-Based Pest Control
Weilin Zhang

15. Functional Characterization of Target Genes Associated with Insecticide Resistance of the Green Peach Aphid, Myzus persicae
John Fosu-Nyarko, Sadia Iqbal, Frances Brigg, and Michael G.K. Jones

16. Plant-Mediated RNA Interference Expressing dsRNA in Cytoplasm for RNAi-Based Pest Control
Weilin Zhang

17. RNAi Expression in Cotton for Control of Herbivorous Insects
Ricardo Salvador, Laura Maskin, José Niz, Mariana Turica, Analía Pedarros, Esteban Hopp, and Dalia Lewi

18. Transplastomic Tomato Plants Expressing Insect-Specific Double-Stranded RNAs: A Protocol Based on Biolistic Transformation
Emine Kaplanoglu, Igor Kolotilin, Rima Menassa, and Cam Donly

19. Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri
Yulica Santos-Ortega and Alex Flynt

20. Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115 (DE3) for Non-Transgenic RNAi-Based Insect Pest-Management
Mabel L. Taracena, Isabella Garcia Caffaro, Gabriela O. Paiva-Silva, Pedro L. Oliveira, Pedro A. Rendon, Ellen M. Dotson, and Pamela M. Pennington

21. Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects
Paul Dyson, Marcela Figueiredo, Awawing A. Andongma, and Miranda M. A. Whitten

22. Application of Nanoparticle-Mediated RNAi for Efficient Gene Silencing and Pest Control on Soybean Aphids
Shuo Yan and Jie Shen

23. Methods for Delivery of dsRNAs for Agricultural Pest Control: The Case of Lepidopteran
Bruna Garbatti Factor, Flavia de Moura Manoel Bento, and Antonio Figueira

24. The CRISPR/Cas9-Mediated Genome Editing System in Insect Genomics and Pest Management
Rihui Yan and Xianwu Lin

Subject Index List…