RNA Interference in insect cells infected with baculovirus

ISBN-13: 9783843351355 / Angielski / Miękka / 2010 / 144 str.

Tamer Salem

Opis

Baculovirus-insect cell system (BICS) is considered one of the most efficient eukaryotic gene expression systems. Establishing a universal gene silencing (UGS) system is very important due to the increasing number of studies using RNA interference (RNAi) with BICS. In this work, the enhanced green fluorescent protein (EGFP) was used as the RNAi consistent target sequence located downstream of a depressant insect-neurotoxin gene, LqqIT2 used as a model of the gene of interest. Small interfering RNA (siRNA) and inverted repeats of EGFP gene (IR-EG) were examined in targeting the EGFP-LqqIT2 (EL)-fusion mRNA or LqqIT2-EGFP (LE)-bicistronic mRNA for degradation. Suppression efficiencies using these inducers were examined transiently and stably in uninfected and infected insect Sf9 cells.

O autorze

Tamer Salem is a Research Associate Professor in Molecular Virology. He is working in AGERI, Egypt. He obtained his Ph.D. from Microbiology Department in University of Florida, USA. He had varies post- doctoral and research associate positions in Miami University (Ohio), University of Florida (Florida), and Michigan State University (East Lansing).