RNA Interference: Challenges and Therapeutic Opportunities

ISBN-13: 9781493915378 / Angielski / Twarda / 2014 / 495 str.

Mouldy Sioud

Opis

RNA Interference: Challenges and Therapeutic Opportunities provides readers with recent advances in siRNA design, delivery, targeting and methods to minimize siRNA's unwanted effects. Preclinical and clinical use of synthetic siRNAs, the roles of miRNAs in cancer and the promise of extracellular miRNAs for diagnosis are also covered in this meticulous collection, along with novel methods for identifying endogenous siRNAs and the annotation of small RNA transcriptomes. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.Comprehensive and cutting-edge, RNA Interference: Challenges and Therapeutic Opportunities will aid researchers, clinicians, teachers and biotechnologists interested in the power of RNA-based therapies.

Spis treści

1. RNA Interference: Mechanisms, Technical Challenges, and Therapeutic Opportunities

            Mouldy Sioud

 

2. Nanostructured RNAs for RNA Interference

            Yuko Nakashima, Naoko Abe, Yoshihiro Ito, and Hiroshi Abe

 

3. One Long Oligonucleotide or Two Short Oligonucleotides Based shRNA Construction and Expression

            Xue-jun Wang and Sheng-qi Wang

 

4. PLGA Microspheres Encapsulating siRNA

            Giuseppe De Rosa and Giuseppina Salzano

 

5. Magnetic Nanoparticle and Magnetic Field Assisted siRNA Delivery In Vitro

            Olga Mykhaylyk, Yolanda Sanchez-Antequera, Dialechti Vlaskou, Maria Belen Cerda, Mehrdad Bokharaei, Edelburga Hammerschmid, Martina Anton, and Christian Plank

 

6. Cytoplasmic Delivery of siRNAs to Monocytes and Dendritic Cells Via Electroporation

            Mouldy Sioud

 

7. Delivery of siRNAs to Cancer Cells Via Bacteria

            Omar Ahmed, Andrea Krühn, and Hermann Lage

 

8. Microwell Array-Mediated Delivery of Lipoplexes Containing Nucleic Acids for Enhanced Therapeutic Efficacy

            Yun Wu, Daniel Gallego-Perez, and L. James Lee

 

9. Modulating the Tumor Microenvironment with RNA Interference as a Cancer Treatment Strategy

            Karin Zins, Mouldy Sioud, Seyedhossein Aharinejad, Trevor Lucas, and Dietmar Abraham

 

10. Targeted In Vivo Delivery of siRNA and an Endosome-Releasing Agent to Hepatocytes

            Magdolna G. Sebestyén, So C. Wong, Vladimir Trubetskoy, David L. Lewis, and Christine I. Wooddell

 

11. Cell-Internalization SELEX: Method for Identifying Cell-Internalizing RNA Aptamers for Delivering siRNAs to Target Cells

            William H. Thiel, Kristina W. Thiel, Katie S. Flenker, Tom Bair, Adam J. Dupuy, James O. McNamara II, Francis J. Miller, and Paloma H. Giangrande

 

12. Strategies for siRNA Navigation to Desired Cells

            Mouldy Sioud

 

13. Analysis of RNA Interference-Mediated Hepatic Gene Silencing Following Murine Hydrodynamic Injection of Guanidinopropyl-Modified siRNAs

            Maximilian C.R. Buffb, Stefan Bernhardt, Musa D. Marimani, Abdullah Ely, Joachim W. Engels, and Patrick Arbuthnot

 

14. Therapy of Respiratory Viral Infections with Intranasal siRNAs

            Sailen Barik and Patrick Lu

 

15. Engineering Therapeutic Cancer Vaccines that Activate Anti-Tumor Immunity

            Per Ole Iversen  and Mouldy Sioud

 

16. Immunosuppressive Factor Blockade in Dendritic Cells Via siRNAs Results in Objective Clinical Responses

            Mouldy Sioud, Anne Mobergslien, and Stein Sæbøe-Larssen

 

17. Targeting bcr-abl Transcripts with siRNAs in an Imatinib-Resistant Chronic Myeloid Leukemia Patient: Challenges and Future Directions

            Michael Koldehoff

 

18. A Facile Method for Interfering with Off-Target Silencing Mediated by the Sense Strand

            Anne Mobergslien and Mouldy Sioud

 

19. Overcoming the Challenges of siRNA Activation of Innate Immunity: Design Better Therapeutic siRNAs

            Mouldy Sioud

 

20. Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs

            Jia Han Deng, Peter Deng, Shi-Lung Lin, and Shao-Yao Ying

 

21. In Silico Identification of Novel Endo-siRNAs

            Andrew Schuster, Grant W Hennig, Nicole Ortogero, Dickson Luong, and Wei Yan

 

22. Computer-Assisted Annotation of Small RNA Transcriptomes

            Nicole Ortogero, Grant W. Hennig, Dickson Luong, and Wei Yan

 

23. miR-CATCH: MicroRNA Capture Affinity Technology

            Sebastian Vencken, Tidi Hassan, Noel G. McElvaney, Stephen G.J. Smith, and Catherine M. Greene

 

24. Roles of MicroRNAs in Cancers and Development

            Shigeru Takasaki

 

25. Targeting MicroRNAs to Withstand Cancer Metastasis

            Valentina Profumo, Valentina Doldi, Paolo Gandellini, and Nadia Zaffaroni

 

26. Urinary MicroRNAs as a New Class of Non-Invasive Biomarkers in Oncology, Nephrology, and Cardiology

            Hana Mlcochova, Renata Hezova, Albano C. Meli, and Ondrej Slaby

 

27. Magnetic Bead-Based Isolation of Exosomes

            Morten P. Oksvold, Axel Neurauter, and Ketil W. Pedersen

 

28. Modified 2’-Ribose Small RNAs Function as Toll-Like Receptor-7/8 Antagonists

            Mouldy Sioud