RNA Imaging: Methods and Protocols

ISBN-13: 9781493949748 / Angielski / Miękka / 2016 / 218 str.

Zdravka Medarova

Spis treści

1. Imaging Functional Nucleic Acid Delivery to Skin

            Roger L. Kaspar, Robyn P. Hickerson, Emilio González-González, Manuel A. Flores, Tycho P. Speaker, Faye A. Rogers, Leonard M. Milstone, and Christopher H. Contag

 

2. In Vivo Magnetic Resonance Imaging of Small Interfering RNA Nanodelivery to Pancreatic Islets

            Ping Wang and Anna Moore

 

3. Magnetic Resonance Spectroscopy of siRNA-Based Cancer Therapy

            Marie-France Penet, Zhihang Chen, Noriko Mori, Balaji Krishnamachary, and Zaver M. Bhujwalla

 

4. Targeted Delivery with Imaging Assessment of siRNA Expressing Nanocassettes into Cancer

            Wei Chen and Lily Yang

 

5. Analyses of Tumor Burden In Vivo and Metastasis Ex Vivo Using Luciferase-Expressing Cancer Cells in an Orthotopic Mouse Model of Neuroblastoma

            Frances L. Byrne, Joshua A. McCarroll, and Maria Kavallaris

 

6. Indium-Labeling of siRNA for Small Animal SPECT Imaging

            Steven Jones and Olivia Merkel

 

7. Imaging of Electrotransferred siRNA

            Muriel Golzio and Justin Teissié

 

8. Whole-Body Scanning PCR, a Tool for the Visualization of the In Vivo Biodistribution Pattern of Endogenous and Exogenous Oligonucleotides in Rodents

            Julien A. Boos and Iwan Beuvink

 

9. siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo

            Seung Koo Lee

and Ching-Hsuan Tung

 

10. Sensing miRNA: Signal Amplification by Cognate RISC for Intracellular Detection of miRNA in Live Cells

            Amol Kavishwar and Zdravka Medarova

 

11. Molecular Beacon-Based MicroRNA Imaging During Neurogenesis

            Jonghwan Lee and Soonhag Kim

 

12. Hypoxia-Responsive Copolymer for siRNA Delivery

            Federico Perche, Swati Biswas, Niravkumar R. Patel, and Vladimir P. Torchilin

 

13. Controlling RNA Expression in Cancer Using Iron Oxide Nanoparticles Detectable by MRI and In Vivo Optical Imaging

            Zdravka Medarova, Mustafa Balcioglu, and Mehmet V. Yigit

 

14. Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures

            Karmele Valencia and Fernando Lecanda

 

15. Positive Bioluminescence Monitoring of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES

            Patrick Baril and Chantal Pichon

 

16. MicroRNA Imaging in Combination with Diagnostic Ultrasound and Bubble Liposomes for MicroRNA Delivery

            Yoko Endo-Takahashi, Yoichi Negishi, Ryo Suzuki, Kazuo Maruyama, and Yukihiko Aramaki

Blurb

This detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques for in vivo micro/siRNA imaging including the design and synthesis of specific imaging agents and tools, the development of imaging methodologies, and their interpretation. An essential element in the development and optimization of these therapies is the ability to measure the bioavailability and functionality of the RNA/oligonucleotide molecule after administration into the body. Noninvasive imaging provides the necessary set of tools to accomplish this in authentic physiologic environments and across time. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, RNA Imaging: Methods and Protocols serves physicians, scientists, and graduate students who are either new to the field of RNA-based imaging and its associated therapeutic applications or who wish to be apprised of recent advances in the state of the art.