REGULATION OF PP1 2 IN TESTIS/SPERMATOZOA BY PPP1R11/PPP1R7/PPP1R2 : The Macromolecular Complex Formed by PP1gamma2 in Male Germ Cells

ISBN-13: 9783639141559 / Angielski / Miękka / 2009 / 120 str.

Lina Cheng

Opis

In mouse testis, PP1 1 is restricted mainly tosomatic cells, while PP1 2 is primarily located in secondary spermatocytes andgerm cells, and the only isoform detectable in mammalian spermatozoa.Targeted deletion of the PP1 gene results in sterile male mice.Because the most prominent marker of this phenotype is aberrant spermatid morphogenesis, its primary cause may be the absenceof PP1 2 since PP1 1 is mainly in somatic cells. To betterunderstand the role of PP1 2 in mammalian testis/sperm, this dissertationwas undertaken to explore PP1 2 interactions with its regulatoryproteins in testis/sperm. Wild-type and mutant isoforms of PPP1R11/inhibitor 3(I3), PPP1R2/inhibitor 2 (I2) and a novel protein similarto I2 were studied. The studies from this dissertation provide importantinsights into the regulation of PP1 2 in testis and sperm.

O autorze

I got my MD degree from Shanxi Medical University, China, in
1998, Master degree in
Neurobiology from Shanghai Medical Center of Fudan University
(former: Shanghai
Medical University), China, in 2001, and Ph.D. in Reproductive
Physiology at Kent State
University, USA, in 2008.