Quantum Dots: Applications in Biology

ISBN-13: 9781588295620 / Angielski / Twarda / 2007 / 257 str.

Charles Z. Hotz; Charles Z. Hotz; Marcel P. Bruchez

Opis

Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.

Recenzje

From the reviews:

"...provides an excellent reference for protocols that can be utlized repeatedly to yield reliable performance from quantum dot-enabled applications." -Anticancer Research 27, May 2007

"Bruschez and Holtz have compiled an extremely useful set of applications and protocols in Quantum Dots, Applications in Biology. The protocols, written by the many leaders in QD imaging, cover a wide range of applications and provide detailed methodology for each application supported by excellent illustrations. This book certainly belongs on the shelf of anyone interested in biological imaging. ... in summary, this illustrative compilation of well-written protocols on QD is a must have for both the expert and novice interested in biological imaging." (Neel Ghosh, ChemBioChem, Issue 9, 2008)

Spis treści

Table of Contents Preface Contributors Part 1 - Imaging of fixed cells and tissues 1. Immunofluorescent Labeling of Proteins in Cultured Cells with Quantum Dot Secondary Antibody Conjugates Richard L. Ornberg and Hongjian Liu 2. Immunohistochemical Detection with Quantum Dots Rizwan S. Akhtar, Cecelia B. Latham, Dario Siniscalco, Carlo Fuccio and Kevin A. Roth 3. Quantum Dots for Multicolor Clinical Pathology and Multispectral Imaging Johbu Itoh and Robert Yoshiyuki Osamura 4. Light and Electron Microscopic Localization of Multiple Proteins using Quantum dots Thomas J. Deerinck, Ben N. G. Giepmans, Benjamin L. Smarr, Maryann E. Martone and Mark H. Ellisman 5. Human Metaphase Chromosome FISH using Quantum Dot Conjugates Joan H.M. Knoll Part 2 - Imaging of live cells 6. Biotin-Ligand Complexes with Streptavidin Quantum Dots for in vivo Cell Labeling of Membrane Receptors Diane S. Lidke, Peter Nagy, Thomas M. Jovin and Donna J. Arndt-Jovin 7. Single Quantum Dot Tracking of Membrane Receptors Cédric Bouzigues, Sabine Lévi, Antoine Triller and Maxime Dahan 8. Optical Monitoring of Single Cells Using Quantum Dots Jyoti K. Jaiswal and Sanford M. Simon 9. Peptide Mediated Intracellular Delivery of Quantum Dots B. Christoffer Lagerholm 10. High Content Analysis in Multiple Cell Lines Using Quantum Dots Paul G. Wylie 11. Measuring Cell Motility Using Quantum Dot Probes Weiwei Gu, Teresa Pellegrino, Wolfgang J. Parak, Rosanne Boudreau, Mark A. Le Gros, A. Paul Alivisatos and Carolyn A. Larabell Part 3 - Imaging of live animals 12. Quantum Dots for In-vivo Molecular and Cellular Imaging Xiaohu Gao, Leland WK Chung and Shuming Nie 13. Sentinel Lymph Node Mapping with Type II Quantum Dots John V. Frangioni, Sang-WookKim, Shunsuke Ohnishi, Sungjee Kim and Moungi Bawendi 14. Macrophage-mediated Co-localization of Quantum Dots in Experimental Glioma Osman Muhammad, Alexandra Popescu and Steven A. Toms Part 4 - Flow cytometry applications 15. Applications of Quantum Dots to Multicolor Flow Cytometry Pratip K. Chattopadhyay and Mario Roederer 16. Quantum Dots in Flow Cytometry Barnaby Abrams and Tim Dubrovsky Part 5 - Biochemical applications 17. Luminescent Biocompatible Quantum Dots: A tool for immunosorbent assay design Ellen R. Goldman, H. Tetsuo Uyeda, Andrew Hayhurst, and Hedi Mattoussi 18. Fluorescence-based Analysis of Cellular Protein Lysate Arrays Using Quantum Dots David H. Geho, J. Keith Killian, Animesh Nandi, Johanne Pastor, Prem Gurnani and Kevin P. Rosenblatt. 19. Application of Quantum Dots to Multi-Color Microarray Experiments: Four-Color Genotyping George Karlin-Neumann, Marina Sedova, Mat Falkowski, Zhiyong Wang, Steven Lin and Maneesh Jain Index