Quantum Dots: Applications in Biology

ISBN-13: 9781493912797 / Angielski / Twarda / 2014 / 258 str.

Adriana Fontes; Beate Saegesser Santos

Opis

In Quantum Dots: Applications in Biology, Second Edition, expert researchers in the field detail consolidated approaches as well as new trends in the field. Organized into five parts, the first part comprises an introduction on Quantum Dots (QDs) as fluorescent probes in Life Sciences. While the second section covers important features about QDs preparative processes and characterizations for their successful application as fluorophores. The third part presents main aspects related to QDs methods applied to live cells and tissues. The fourth section focuses on QDs experiments in small animals and the fifth part demonstrates the versatility of QDs in a set of FRET applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Thorough and intuitive, In Quantum Dots: Applications in Biology, Second Edition aids scientists in continuing to study QDs by providing information about methods and protocols helping to expand their research.

Spis treści

Part I – Introduction to Quantum Dots

1. Quantum Dots as Biophotonics Tools

Carlos Lenz Cesar

 

Part II – Quantum Dots’ Preparative Processes and Characterizations

2. Photoligation Combined with Zwitterion-Modified Lipoic Acid Ligands Provides Compact and Biocompatible Quantum Dots

Naiqian Zhan, Goutam Palui, Henry Grise, and Hedi Mattoussi

 

3. Analysis of Quantum Dots and Their Conjugates by Capillary Electrophoresis with Detection of Laser-Induced Luminescence

 Karel Klepárník, Vladimíra Datinská, Ivona Voráčová, and Marcela Lišková

 

4. Advanced Procedure for Oriented Conjugation of Full-Size Antibodies with Quantum Dots

Kristina Brazhnik, Igor Nabiev, and Alyona Sukhanova

 

5. Quantum Dots-Antibody Conjugates via Carbodiimide-Mediated Coupling for Cellular Imaging

Daniel Alistair East, Michael Todd, and Ian James Bruce

 

6. Measuring the Hydrodynamic Radius of Quantum Dots by Fluorescence Correlation Spectroscopy

André A de Thomaz, Diogo B. Almeida, and Carlos Lenz Cesar

 

7. Quantum Dots Fluorescence Quantum Yield Measured by Thermal Lens Spectroscopy

Carlos Estupiñán-López, Christian Tolentino Dominguez, Paulo. E. Cabral Filho, Adriana Fontes, Renato E. de Araujo

 

8. Semiquantitative Fluorescence Method for Bioconjugation Analysis

Aluízio G. Brasil Jr., Kilmara H. G. Carvalho, Elisa S. Leite, Adriana Fontes, Beate S. Santos

 

Part III – Quantum Dots for Live Cells and Tissues Applications

9. Assembly, Characterization and Delivery of Quantum Dot Labeled Biotinylated Lipid Particles

Valeria Sigot

 

10. Oriented Conjugation of Single-Domain Antibodies and Quantum Dots

Kristina Brazhnik, Igor Nabiev, and Alyona Sukhanova

 

11. Functional Integration of Quantum Dot Labeled Mesenchymal Stem Cells in a Cardiac Microenvironment

Maria C. Collins, Philip R. Gunst, and Barbara J. Muller-Borer

 

12. In Vitro Approaches to Assessing the Toxicity of Quantum Dots

Ryan S. McMahan, Vivian Lee, William C. Parks, Terrance J. Kavanagh, and David L. Eaton

 

13. Basics for the Preparation of Quantum Dots and their Interactions with Living Cells

Xiue Jiang, Jing Bai, and Tiantian Wang

 

Part IV – QDs for Small Animals Applications

14. In Vivo Approaches to Assessing the Toxicity of Quantum Dots

David K Scoville, Christopher M Schaupp, Francois Baneyx,  and Terrance J Kavanagh

 

15. Quantum Dots for Imaging Neural Cells In Vitro and In Vivo

Angela O. Choi, Kevin D. Neibert, and Dusica Maysinger

 

16. A Short-Term Inhalation Study Protocol – Designed for Testing of Toxicity and Fate of Nanomaterials

Lan Ma-Hock, Thomas Hofmann, Robert Landsiedel, and Bennard van Ravenzwaay

 

Part V – Quantum Dots for FRET Applications

17. Quantitative Measurement of Proteolytic Rates with Quantum Dot-Peptide Substrate Conjugates and Förster Resonance Energy Transfer

Miao Wu, Eleonora Petryayeva, Igor L. Medintz, and W. Russ Algar

 

18. Solid-Phase Supports for the in situ Assembly of Quantum Dot-FRET Hybridization Assays in Microfluidics

Anthony J. Tavares, M. Omair Noor, Uvaraj Uddayasankar, Ulrich J. Krull, and Charles H. Vannoy