Quantitative Trait Loci (Qtl): Methods and Protocols

ISBN-13: 9781493958320 / Angielski / Miękka / 2016 / 331 str.

Scott A. Rifkin

Opis

This Methods in Molecular Biology(TM) volume offers techniques for the entire process of quantitative train loci experiments: the mapping populations, identifying quantitative trait loci, extending the power of quantitative trait locus analysis and case studies.

Spis treści

Part I. Setting Up Mapping Populations

 

1. Backcross Populations and Near Isogenic Lines

Rik Kooke, Erik Wijnker and Joost. J.B. Keurentjes

 

2. F₂ Designs for QTL Analysis

Yuan-Ming Zhang

 

3. Design and Construction of Recombinant Inbred Lines

Daniel A. Pollard

 

4. Two Flavors of Bulk Segregant Analysis in Yeast

Maitreya J. Dunham

 

5. Selecting Markers and Evaluating Coverage

Matthew A. Cleveland and Nader Deeb

 

Part II. Identifying Quantitative Trait Loci

 

6. Composite Interval Mapping and Multiple Interval Mapping: Procedures and Guidelines for Using Windows QTL Cartographer

Luciano Da Costa E Silva, Shengchu Wang, and Zhao-Bang Zen

 

7. Design Database for Quantitative Trait Loci (QTL) Data Warehouse, Data Mining and Meta Analysis

Zhi-Liang Hu, James M Reecy and Xiao-Lin Wu

 

8. Meta-Analysis of QTL Mapping Experiments

Xiao-Lin Wu and Zhi-Liang Hu

 

Part III. Extending the Power of Quantitative Train Locus Analysis

 

9. Using eQTLs to Reconstruct Gene Regulatory Networks

Lin S. Chen

 

10. Estimation and Interpretation of Genetic Effects with Epistasis using the NOIA Model

José M Álvarez-Castro, Örjan Carlborg and Lars Rönnegård

 

11. Identifying QTL for Multiple Complex Traits in Experimental Crosses

Samprit Banerjee and Nengjun Yi

 

12. Functional Mapping of Developmental Processes: Theory, Applications and Prospects

Kiranmoy Das, Zhongwen Huang, Jingyuan Liu, Guifang Fu, Jiahan Li, Yao Li, Chunfa Tong, Junyi Gai and Rongling Wu

 

13. Statistical Models for Genetic Mapping in Polyploids: Challenges and Opportunities

Jiahan Li, Kiranmoy Das, Guifang Fu, Yao Li, Christian Tobias and Rongling Wu

 

Part IV. Case Studies

 

14. eQTL

Lun Li, Xianghua Zhang and Hongyu Zhao

 

15. Genetic Mapping of Quantitative Trait Loci for Disease-Related Phenotyples

Marcella Devoto and Mario Falchi

 

16. Quantitative Trait Locus Analysis in Haplodiploid Hymenoptera

Jürgen Gadau, Christof Pietsch, Leo W Beukeboom

Blurb

Over the last two decades advances in genotyping technology, and the development of quantitative genetic analytical techniques have made it possible to dissect complex traits and link quantitative variation in traits to allelic variation on chromosomes or quantitative trait loci (QTLs). In Quantitative Trait Loci (QTLs):Methods and Protocols, expert researchers in the field detail methods and techniques that focus on specific components of the entire process of quantitative train loci experiments. These include methods and techniques for the mapping populations, identifying quantitative trait loci, extending the power of quantitative trait locus analysis, and case studies. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Thorough and intuitive, Quantitative Trait Loci (QTLs):Methods and Protocols aids scientists in the further study of the links between phenotypic and genotypic variation in fields from medicine to agriculture, from molecular biology to evolution to ecology.