Public Health Microbiology: Methods and Protocols

ISBN-13: 9781617373718 / Angielski / Miękka / 2010 / 548 str.

John F. T. Spencer;Alicia L. Ragout De Spencer

Opis

Public Health Microbiology: Methods and Protocols is focused on microorganisms that can present a hazard to human health in the course of everyday life. There are chapters dealing with organisms that are directly pathogenic to humans, including bacteria, viruses, and fungi; on organisms that produce toxins during growth in their natural habitats; on the use of bacteriocins produced by such organisms as lactobacilli and bifidobacteria; as well as several chapters on hazard analysis, the use of disinfectants, microbiological analysis of cosmetics, and microbiological tests for sanitation equipment in food factories. Additional chapters look at the use of animals (mice) in the study of the various characteristics of milk and their relationships with lactic acid bacteria in particular. Other chapters focus on special methods for determining particular components of milk. In particular, in Parts I and II, on bacterial and viral pathogens, special attention is given to methods for PCR detection of genes with resistance to tetracycline, as well as to Salmonella enterica; for identification and typing of Campylobacter coli; for detection of the abundance of enteric viruses, hepatitis A virus, and rotaviruses in sewage, and of bacteriophages infecting the O157: H7 strain of Escherichia coli. Part III offers methods for computerized analysis and typing of fungal isolates, for isolation and enumeration of fungi in foods, and for the determination of aflatoxin and zearalenone

Recenzje

"...highly recommended for graduate students as well as advanced researchers working in the field of public health microbiology." - International Journal of Hygiene and Environmental Health

Spis treści

Part I. Bacteria Detection of Tetracycline Resistance Genes by PCR Methods Rustam I. Aminov, Joanne C. Chee-Sanford, Natalie Garrigues, Asma Mehboob, and Roderick I. Mackie Integron Analysis and Genetic Mapping of Antimicrobial Resistance Genes in Salmonella enterica serotype Typhimurium Mairéad Daly and Séamus Fanning Molecular-Based Identification and Typing of Campylobacter jejuni and C. coli Brigid Lucey, Fiona O'Halloran, and Séamus Fanning Molecular Genotyping Methods and Computerized Analysis for the Study of Salmonella enterica Ana Belén Vivanco, Juan Alvarez, Idoia Laconcha, Nuria López-Molina, Aitor Rementeria, and Javier Garaizar Part II. Viruses Detection of Infectious Rotaviruses by Flow Cytometry Albert Bosch, Rosa M. Pintó, Jaume Comas, and Francesc-Xavier Abad Integrated Cell Culture/PCR for Detection of Enteric Viruses in Environmental Samples Kelly A. Reynolds Abundance in Sewage of Bacteriophages Infecting Escherichia coli O157:H7 Maite Muniesa and Juan Jofre Molecular Genotyping of Irish Rotavirus Strains Fiona O'Halloran and Séamus Fanning Hepatitis A Virus: Molecular Detection and Typing Glòria Sánchez, Cristina Villena, Albert Bosch, and Rosa M. Pintó Part III. Fungi Typing Fungal Isolates: Molecular Methods and Computerized Analysis Aitor Rementeria, Ana Belén Vivanco, Ainara Cadaval, María Teresa Ruesga, Sonia Brena, José Pontón, Guillermo Quindós, and Javier Garaizar Fungal Isolation and Enumeration in Foods Dante Javier Bueno, Julio Oscar Silva, and Guillermo Oliver Determination of Aflatoxins and Zearalenone in Different Culture Media Dante Javier Bueno and Guillermo Oliver Part IV. Other Pathogens Intracellular Multiplication of Legionella Species and the Influence of Amoebae on TheirIntracellular Growth in Human Monocytes: Mono Mac 6 Cells and Acanthamoeba castellanii as Suitable In Vitro Models Birgid Neumeister Viability of Amoebae, Fungal Conidia, and Yeasts: Rapid Assessment by Flow Cytometry Judith A. Noble-Wang, Shangtong Zhang, Daniel Price, and Donald G. Ahearn Detection and Differentiation of Cryptosporidium Oocysts in Water by PCR-RFLP Lihua Xiao, Altaf A. Lal, and Jianlin Jiang Genotyping of Cryptosporidium parvum With Microsatellite Markers Giovanni Widmer, Xiaochuan Feng, and Sultan Tanriverdi Immunomagnetic Separation of Pathogenic Organisms From Environmental Matrices Gary P. Yakub and Kathleen L. Stadterman-Knauer Detection of Erysipelothrix rhusiopathiae in Clinical and Environmental Samples Silvana G. Fidalgo and Thomas V. Riley Interaction Between Lactic Acid Bacteria and Gastrointestinal Nematodes of Caprine Origin Diana Draksler, María Cecilia Monferran, and Silvia González Molecular Detection of Genes Responsible for Cyanobacterial Toxin Production in the Genera Microcystis, Nodularia, and Cylindrospermopsis Brendan P. Burns, Martin L. Saker, Michelle C. Moffitt, and Brett A. Neilan Part V. Bacteriocins and Other Inhibitors Purification of Antilisterial Bacteriocins Jean-Marc Berjeaud and Yves Cenatiempo The Hazard Analysis and Critical Control Point System in Food Safety Anavella Gaitan Herrera Testing Disinfectants in the Food Factory: Phenol Coefficient Method Anavella Gaitan Herrera Nontraditional Method of Evaluating Disinfectants: With Isolated Microorganisms From the Food Factory Anavella Gaitan Herrera Microbiological Analysis of Cosmetics Anavella Gaitan Herrera Helicobacter pylori and Food Products: A Public Health Problem Anavella Gaitan Herrera Microbiological Test for

Blurb

The world abounds with microorganisms that are hazardous to human health in the normal course of life. In Public Health Microbiology: Methods and Protocols, expert investigators describe in step-by-step detail the laboratory techniques they have perfected for determining the nature and character of a diverse group of hazardous bacteria, viruses, and fungi. For bacterial and viral pathogens, the authors give special attention to PCR methods for detecting genes resistant to tetracycline, for resistance in Salmonella enterica, for identifying and typing Campylobacter coli, and for detecting the abundance of enteric, hepatitis A, and rotaviruses in sewage, as well as bacteriophages infecting the O157:H7 strain of Escherichia coli. For fungi the authors offer methods for computerized analysis and typing of fungal isolates, for the isolation and enumeration of fungi in foods, and for the determination of aflatoxin and zearalenone. Other methods deal with hazard analysis, the use of disinfectants, microbiological analysis of cosmetics, microbiological tests for sanitation equipment in factories, and the uses of animals in studies of various characteristics of lactobacilli. Review articles address the spread of pathogens from livestock and poultry production, including the threat of prion-based diseases. All protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Timely and highly practical, Public Health Microbiology: Methods and Protocols offers researchers and public health specialists alike a wide-ranging collection of methods for the determination of many important pathogenic microorganisms, their effects on health, and possible measures that can be taken to counter these effects.