Protoplast Technology: Methods and Protocols

ISBN-13: 9781071621660 / Angielski

Kan Wang; Feng Zhang

Opis

This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.

Spis treści

Part I: Basic Techniques and Their Applications

 

1. Physcomitrium patens Protoplasting and Protoplast Transfection

            Florence Charlot, Guillaume Goudounet, Fabien Nogué, and Pierre-François Perroud

 

2. Protoplast Isolation and Transfection in the Single-Cell C₄ Species Bienertia sinuspersici

            Shiu-Cheung Lung, Nilanth Yogadasan, Makoto Yanagisawa, and Simon D.X. Chuong

 

3. Protoplast for Gene Functional Analysis in Arabidopsis

            Yanlin Liu and Yan Xiong

 

4. Application of Protoplast Regeneration to CRISPR/Cas9 Mutagenesis in Nicotiana tabacum

            Fu-Hui Wu, Yu-Hsuan Yuan, Chen-Tran Hsu, Qiao-Wei Cheng, and Choun-Sea Lin

 

5. CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration

            Raviraj Banakar, Krishan M. Rai, and Feng Zhang

 

6. Protoplast Isolation and Transfection in Rice

            Zhaohui Zhong and Yong Zhang

 

7. Protoplast Isolation and Transfection in Maize

            Monique R. Coy, Shane E. Abbitt, and Mary J. Frank

 

8. Isolation and Transfection of Maize Endosperm Protoplasts

            Yufeng Hu and Yubi Huang

 

9. Protoplast Isolation from Undifferentiated Maize Seedling Shoot Tissue

            James W. Satterlee and Michael J. Scanlon

 

10. Protoplast Isolation and Transfection in Wheat

            Guangbin Luo, Boshu Li, and Caixia Gao

 

11. Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses

            Zeng-Yu Wang and German Spangenberg

 

12. Efficient Protoplast Isolation and DNA Transfection for Winter Oilseed Crops, Pennycress (Thlaspi arvense) and Camelina (Camelina sativa)

            Minjeong Kang, Keunsub Lee, and Kan Wang

 

13. Protoplast Isolation, Transfection, and Gene Editing for Soybean (Glycine max)

            Gunvant B. Patil, Robert M. Stupar, and Feng Zhang

 

14. Protoplast Isolation and Transformation in Oil Palm

            Mat Yunus Abdul Masani, Ghulam Kadir Ahmad Parveez, Gundula Noll, MD Piji Mohd Al Akmarul Fizree, Ravigadevi Sambanthamurthi, and Dirk Pruefer

 

Part II: Advanced Systems for High Throughput Analysis and Automation

 

15. Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant Cells

            Ward Decaestecker, Norbert Bollier, Rafael A. Buono, Moritz K. Nowack, and Thomas B. Jacobs

 

16. Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis via Protoplast Transfection

            Adam Sychla, Juan Armando Casas-Mollano, Matthew H. Zinselmeier, and Michael Smanski

 

17. High-Throughput Transfection and Analysis of Soybean (Glycine max) Protoplasts

            Alessandro Occhialini, Mary-Anne Nguyen, Lezlee Dice, Alexander C. Pfotenhauer, Mst Shamira Sultana, C. Neal Stewart, Jr, and Scott C. Lenaghan