Protocols for the Diagnosis of Pig Viral Diseases

ISBN-13: 9781071620427 / Angielski / Twarda / 2022

Opis

This detailed book explores new molecular biological techniques for the detection of both antigens and antibodies of porcine diseases. Assays examined within this collection include improved methods of nucleic acid extraction, polymerase chain reaction (PCR), droplet digital PCR (ddPCR), polymerase spiral reaction (PSR), cross-priming amplification (CPA), enzyme-linked immunosorbent assay (ELISA), as well as peptide nucleic acid (PNA) based tools, aptamer-based tools, and lateral flow assays and different immune assay-based diagnostics for porcine diseases. As part of the Springer Protocols Handbooks series, the present book contains the type of in-depth knowledge on important diagnostics tools that provide a vital source of information for scientists, researchers, and students from different fields. 

Authoritative and practical, Protocols for the Diagnosis of Pig Viral Diseases aims to guide researchers toward developing new technologies for the diagnosis of pig pathogens.

Spis treści

1. Requirements and Preparedness for Attending a Viral Disease Outbreak in Pig Farms

            Dipak Deka, Pankaj Kumar Dhaka, Ujjwal Kumar De, Obli Rajendran Vinodh Kumar, and Yashpal Singh Malik

 

2. Collection of Samples, Their Preservation, and Transportation

            Ashok Kumar, Kaushal Kishor Rajak, Ajay Kumar Yadav, Vishal Rai, Mukesh Bhatt, R.P. Singh, and R.K. Singh

 

3. Methods for Quantification of Viruses

            Mukesh Bhatt, Chris Einstein, Kiran, Arfa Fayaz, Vishal Rai, Monu Karki, Ashok Kumar, Ajay Kumar Yadav, and Kaushal Kishor Rajak

 

4. Protocols for Isolation of Genetic Materials from RNA Viruses

            Nihar Nalini Mohanty, Vikas Gupta, Laxmi Narayan Sarangi, Rohini Bhat, and Sathish B. Shivachandra

 

5. Multiplex PCR for Diagnosis of Porcine Diseases

            Manjisha Choudhury, Ajay Kumar Yadav, Seema Rani Pegu, Rajib Deb, and Swaraj Rajkhowa

 

6. Protocols for Isolation of Plasmid DNA

            Vinod Kumar Singh, Vikas Gupta, and Chayanika Das

 

7. Recombinant Antigen-Based Diagnostic Assays of Pig Viral Diseases

            Rajib Deb, Ajay Kumar Yadav, Gyanendra Singh Sengar, Seema Rani Pegu, Souvik Paul, Swaraj Rajkhowa, and Vivek Kumar Gupta

 

8. RNA-PAGE-Based Diagnosis of Viral Diseases

            Naveen Kumar, Geetika Kaur, Shubhankar Sircar, Zunjar Dubbal, R.S. Sethi, and Yashpal Singh Malik

 

9. Peptide Nucleic Acid (PNA): A Diagnostic Molecule for Infectious Diseases

            Vinay G. Joshi, Anu Kumari, Sushila Maan, Tarun Kumar, and Satish Kumar

 

10. Nucleic Acid Sequence-Based Amplification (NASBA) Methods and CRISPR/Cas13 System to Detect Pig Viral Diseases

            Ajay Kumar Singh, Soumen Naskar, Pramod W. Ramteke, and Rohit Kumar

 

11. Aptamers as Diagnostic Markers for Viral Infections of Veterinary Importance

            Victoria C. Khangembam and Dimpal Thakuria

 

12. Antibody-Based Sensors for Pathogen Detection

            Nirmita Dutta, Akhil Kumar, Anu Kumari, Sushila Maan, Gorachand Dutta, and Vinay G. Joshi

 

13. Lateral Flow Assay for Diagnosis of Pig Viral Diseases

            Aditya Prasad Sahoo and Rajib Deb

 

14. Droplet Digital PCR-Based Diagnosis for Porcine Viral Diseases

            Yoya Vashi and Sachin Kumar

 

15. Protocols for Immunofluorescence Techniques

            Deepika Bisht, Shikha Saxena, Nitish Singh Kharayat, and Siddharth Gautam

 

16. Polymerase Spiral Reaction (PSR) for the Diagnosis of Porcine Viral Diseases

            Vikas Gupta, Nihar Nalini Mohanty, and Vinod Kumar Singh

 

17. Recombinase Polymerase Amplification-Based Diagnostics of Porcine Viral Diseases

            Yoya Vashi and Sachin Kumar

 

18. Cell Culture System for Porcine Virus Isolation and Propagation

            Vishal Rai, Kaushal Kishor Rajak, Kiran, Arfa Fayaz, Monu Karki, Chris Einstein, Mukesh Bhatt, Ashok Kumar, and Ajay Kumar Yadav

 

19. An Overview of Mouse Monoclonal Antibody Production

            Kaushal Kishor Rajak, Kiran, Arfa Fayaz, Vishal Rai, Monu Karki, Chris Einstein, Mukesh Bhatt, Ashok Kumar, Ajay Kumar Yadav, and R.P. Singh

 

20. Nucleic Acid Hybridization Techniques for Viral Disease Diagnosis: A Detailed Perspective

            B.V. Sunil Kumar, Himalaya Bhardwaj, Ankita Gurao, Naveen Kumar, and Yashpal Singh Malik

 

21. Ligase Detection Reaction–Fluorescent Microsphere Assay

            A. Raja

 

22. ELISA as a Diagnostic Weapon

            Ramya Kalaivanan and Sankar Palanisamy

 

23. SDS-PAGE and Western Blotting: Basic Principles and Protocol

            Mukesh Bhatt, Vishal Rai, Ashok Kumar, Kiran, Ajay Kumar Yadav, Kaushal Kishor Rajak, Vikas Gupta, Vishal Chander, and R.K. Avasthe

 

24. Immune Assays as Diagnostic for Pig Viral Diseases

            Prabhakar Maurya, Jupi Talukdar, Sarmistha Debbarma, Monuj Kumar Doley, and Luit Barkalita

 

25. Production of Virus-Like Particles Using the Baculovirus Expression System and Their Application in Vaccines and Viral Disease Diagnosis

            Hemanta Kumar Maity, Rajib Deb, Sinéad Lyons, and Ian M. Jones

 

26. Good Laboratory Practices and Biosafety Containments in a Virology Laboratory

            Yashpal Singh Malik, Anuradha Sharma, Niraj Kumar Singh, B.T. Naveen Kumar, and Naveen Kumar

O autorze

Dr. Rajib Deb, PhD is currently employed as Scientist at Indian Council of Agricultural Research-National Research Centre on Pig, Rani, Guwahati, Assam, India. He has served more than 9 years at Agricultural Research Service, India. He completed his post-graduation and PhD at Indian Veterinary Research Institute, Uttar Pradesh, India in the subject of Animal Biotechnology. He is currently pursuing the National Post-Doctoral Fellowship program at National Dairy Research Institute, Karnal, Haryana, India. He has published more than 35 research papers in peer reviewed journals. He is the recipient of several National and International Awards including TWAS Fellowship, Italy; Associate of National Academy of Agricultural Sciences, India; Member of National Academy of Sciences, India; Member of Indian National Young Academy of Sciences, India. He has filed 9 Indian Patents and developed several technologies. He is currently working on the development of diagnostics and vaccines against porcine disease.  

Dr. Ajay Kumar Yadav, PhD is the scientist in veterinary virology at Indian Council of Agricultural Research-National Research Centre on Pig, Rani, Guwahati, Assam, India. He has completed graduation from Govind Ballabh Pant University of Agriculture and Technology, Uttarakhand, India. Later on, he completed his M.V. Sc. and PhD in veterinary virology from the premier veterinary institute named Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, Uttar Pradesh, India. Dr. Yadav joined the Agricultural Research Services (ARS) in the discipline of veterinary microbiology in the year 2018. His research interests include disease diagnosis, cell culture, and virus isolation. He is a member of several societies, and has also published more than 20 research articles in peer-reviewed international and national journals. He has more than 60 GenBank submissions. 

Dr. Swaraj Rajkhowa, PhD joined Agricultural Research Service (ARS) as Scientist in the year 1999. He completed his Ph.D. from Assam Agricultural University in the year 2005. He did his Post-Doctoral studies at internationally reputed institutes like Cornell University, New York, NY, USA and University of California, Irvine, CA, USA where he learned advanced molecular tools like MLST, SLST, SNP, and DNA Microarray technology. His area of interest is molecular epidemiology and molecular diagnosis of infectious diseases of animal origin, including diseases of public health significance. He has been actively involved in diagnosis and management of livestock diseases over the last 20 years and particularly on porcine diseases for the last 12 years. He has published more than 100 research papers in peer reviewed national and international journals of repute, acted as reviewer of several national and international journals, handled more than 10 externally funded projects as Principal Investigator and several institute-funded projects, guided Masters and doctoral degree students (including post-doctoral scholar), and acted as external examiner of both M.V.Sc. and Ph.D. students of SAUs. 

Prof. (Dr.) Yashpal Singh Malik, PhD is serving as Dean, College of Animal Biotechnology at Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India, and previously served as ICAR-National Fellow at Indian Veterinary Research Institute, Izatnagar, India. He works in viral disease epidemiology, virus-host interactions, microbial biodiversity, characterization, and diagnosis of pathogens. Prof. Malik has acquired advanced training in Molecular Virology from the University of Minnesota, Minneapolis, MN, USA; Division of Virology, University of Ottawa, Ottawa, ON, Canada, and Wuhan Institute of Virology, Wuhan, China. He is a recipient of several prestigious National, State, and Academy awards and honors, including the ICAR-Jawaharlal Nehru Award. He has supervised 5 Ph.D. and 17 M.V.Sc. students. Has authored 5 books, 25 book chapters, and published 217 scientific research and review articles. Dr. Malik has been the Editor-in-Chief of Journal of the Immunology Immunopathology. He has also edited special issues of the SpringerNature journal, VirusDisease, Bentham’s journal, Journal of Current Drug Metabolism, on emerging thematic areas.