Proteus Mirabilis: Methods and Protocols

ISBN-13: 9781493996032 / Angielski / Miękka / 2020 / 342 str.

Melanie M. Pearson

Opis

This detailed volume explores essential protocols for the study of Proteus mirabilis which, despite its genetic relatedness to species such as E. coli, often requires specialized handling techniques. This opportunistic bacterial pathogen, most often known as a causative agent of complicated urinary tract infection, is addressed in chapters from global experts in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proteus mirabilis: Methods and Protocols serves as an ideal guide for researchers intrigued by the renewed appreciation for the medical impact and environmental adaptability of this organism, coupled with continued fascination for its dynamic behavior.

Spis treści

1.      Culture methods (obtaining isolated colonies vs swarm; minimal media)

2.      Methods for studying swarming/motility

3.      Cell culture/adherence/invasion/toxins

4.      Urease

5.      UTI mouse model: independent and co-challenge

6.      CAUTI mouse model

7.      Artificial bladder

8.      Catheter biofilms/SEM

9.      Catheter coatings?

10.  LPS analysis (P. mirabilis has at least 79 identified O-serogroups)

11.  Mutagenesis/TargeTron

a.       attTn7 insertion method

12.  Dienes lines/ T6SS

13.  Fimbriae

a.       Culture conditions that favor fimbriation

b.      Shear preps

c.       Acid preps

d.      flow cytometry protocol

14.  IVIS protocol

15.  Clinical

16.  Vaccine protocol

17.  Environmental

18.  Mathematical models

19.  Miscellaneous

a.       Metals (iron, zinc)

b.      Phage

c.       Polymyxin resistance

20.  Techniques that aren’t particularly exclusive to P. mirabilis/UTI

a.       ChIP (PCR and/or seq)

b.      RNA isolation

c.       Microarray analysis

d.      Genome sequencing & analysis

e.      Biofilm assays