Proteoform Identification: Methods and Protocols

ISBN-13: 9781071623244 / Angielski / Twarda / 2022

Sun, Liangliang

Opis

This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS.  

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. Proteoforms and Proteoform Families: Past, Present, and Future 
Lloyd M. Smith

2. Membrane Ultrafiltration-Based Sample Preparation Method and Sheath-Flow CZE-MS/MS for Top-Down Proteomics
Zhichang Yang and Liangliang Sun

3. Size Exclusion Chromatography Strategies and MASH Explorer for Large Proteoform Characterization
Timothy N. Tiambeng, Zhijie Wu, Jake A. Melby, and Ying Ge

4. RPLC-RPLC-MS/MS for Proteoform Identification
Kellye A. Cupp-Sutton, Zhe Wang, Dahang Yu, and Si Wu

5. Monolithic Materials-Based RPLC-MS for Proteoform Separation and Identification
Yu Liang, Lihua Zhang, and Yukui Zhang

6. Capillary Isoelectric Focusing: Mass Spectrometry Method for the Separation and Online Characterization of Monoclonal Antibody Charge Variants at Intact and Subunit Level
Jun Dai, Qiangwei Xia, and Chengjie Ji

7. Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite
Leah V. Schaffer, Michael R. Shortreed, and Lloyd M. Smith

8. Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite
In Kwon Choi and Xiaowen Liu

9. Accurate Proteoform Identification and Quantitation Using pTop 2.0
Rui-Xiang Sun, Rui-Min Wang, Lan Luo, Chao Liu, Hao Chi, Wen-Feng Zeng, and Si-Min He

10. Proteoform Identification and Quantification using Intact Protein Database Search Engine ProteinGoggle
Suideng Qin and Zhixin Tian

11. Mass Deconvolution of Top-Down Mass Spectrometry Datasets by FLASHDeconv
Kyowon Jeong, Jihyung Kim, and Oliver Kohlbacher

12. Deconvolving Native and Intact Protein Mass Spectra with UniDec
Marius M. Kostelic and Michael T. Marty

13. Discovery of Unknown Post-Translational Modifications by Top-Down Mass Spectrometry
Jesse W. Wilson and Mowei Zhou

14. Determining Copper and Zinc Content in Superoxide Dismutase using Electron Capture Dissociation under Native Spray Conditions
Rachel Franklin, Michael Hare, and Joseph S. Beckman

15. Surface-Induced Dissociation for Protein Complex Characterization
Sophie R. Harvey, Gili Ben-Nissan, Michal Sharon, and Vicki H. Wysocki

Subject Index List…