Protein Purification: Design and Scale Up of Downstream Processing

ISBN-13: 9780471037231 / Angielski / Twarda / 1993 / 244 str.

Scott M. Wheelwright;Wheelwright

Opis

This volume describes general techniques useful for translating industrial bioprocesses from the laboratory to the industrial environment. One of the challenges in this field is the integration of engineering and biology in the design of process systems.

Spis treści

Process Design and Competitive Advantage.

Strategies for Process Design.

Protein Characteristics.

Analytical Methods.

Impact of Upstream Operations.

Cell Disruption.

Centrifugation.

Precipitation and Extraction.

Filtration.

General Chromatography.

Ion Exchange Chromatography.

Hydrophobic Interaction Chromatography and Reversed Phase Chromatography.

Affinity Chromatography.

Gel Filtration Chromatography.

Large–Scale Examples.

Index.