Proteases and Cancer: Methods and Protocols

ISBN-13: 9781071635889 / Angielski

Salvatore Santamaria

Spis treści

1. Web-based resources to investigate protease function

Salvatore Santamaria

 

2. N-terminomics/TAILS of human cancer biopsies and cancer cell lines

Afshin Derakhshani, Mitchell Bulluss, Regan Penner, Antoine Dufour

 

3. A new affinity-based probe to profile MMP active forms

Carole Malgorn, François Becher, Pierrick Bruyat, Carole Fruchart-Gaillard, Fabrice Beau, Sarah Bregant, and Laurent Devel

 

4. N-glycan insertion for probing protein-protein interactions and epitope mapping

Shayma Abukar, Xiaohan Zhang, Bertina Dragunaite, Gwladys Chabrier and Rens de Groot

 

5. Expression and purification of recombinant ADAMTS8

Tina Burkhard, Alexander Frederick Minns, and Salvatore Santamaria

 

6. Expression and purification of active monomeric MMP7

Kazuhiro Yamamoto, Moe Isohata, and Shouichi Higashi,

 

7. Mapping the binding sites of MMPs on type II and III collagens using triple-helical peptide Toolkits

Szymon Manka

 

8. Determination of Versikine Levels by Enzyme-Linked Immunosorbent Assay (ELISA)

Alexander Frederick Minns and Salvatore Santamaria

 

9. Methods to investigate thrombin cleavage of osteopontin (OPN)

Lei Zhao, Lawrence L. Leung, and John Morser,

 

10. Using siRNA silencing to analyze ADAM17 in macrophages

Matthew Markham and Linda Troeberg,

 

11. Preparation and characterization of collagen-hyaluronic acid (Col-HA) matrices: in vitro mimics of the tumor microenvironment

Sarbajeet Dutta and Shamik Sen

 

12. Assessment of Invadopodium Formation and Gelatin Degradation In Vitro

Marguerite J. Clarke, Samantha Battagin and Marc G. Coppolino

 

13. Investigation of MT1-MMP activity in cancer cells

Yoshifumi Itoh

 

14. Gelatin in situ zymography to study gelatinase activity in colon cancer cells treated with platelet microparticles (PMPs)

Jakub Kryczka, Hassan Kassassir, Izabela Papiewska-Pająk, and Joanna Boncela,

 

15.  Interrogating the impact of protease activity on tumor progression using 3D spheroid models

Shayin V. Gibson, Edward P. Carter, and Richard P. Grose

 

16. Analysis of Matrix Metalloproteinase Activity and Inhibition in Cancer Spheroids

Anna M. Knapinska, Lillian Onwuha-Ekpete, Gary Drotleff, Destiny Twohill, Cedric Chai, Alexa Ernce, Isabella Grande, Michelle Rodríguez, Dorota Tokmina-Roszyk, Brad Larson, and Gregg B. Fields

 

17. Intravital microscopy to study the effect of matrix metalloproteinases inhibition on Acute myeloid leukemia cells migration in the bone marrow

Floriane S. Tissot, Sara Gonzalez-Anton, and Cristina Lo Celso

 

18. Fluorescence-based peptidolytic assay for high throughput screening of MMP14 inhibitors

Hyun Lee, Lucas Ibrahimi, and Kyu-Yeon Han

 

19. Generation of protease inhibitory antibodies by functional in vivo selection

Ki Baek Lee and Xin Ge

 

20. Engineering selective TIMPs using a counter-selective screening strategy

Hannaneh Ahmadighadykolaei, Evette S. Radisky, and Maryam Raeeszadeh-Sarmazdeh

 

21. Engineering new protease inhibitors using α2-macroglobulin

Seandean Lykke Harwood, and Jan J. Enghild

 

22. Design of bioengineered peptides/proteases as anti-cancer reagents with integrated omics and machine-learning approaches

Weimin Zuoand Hang Fai Kwok

 

Blurb

This volume details bioinformatics analysis, biochemical assays, recombinant protein expression and purification, methods to investigate protease activity in cell-based, organoids and in vivo systems, proteomics, transcriptomics, machine learning and novel approaches to target dysregulated protease activity in cancer.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Proteases and Cancer: Methods and Protocols aims to provide the scientific community with a diverse and representative collection of state-of-the-art methods to investigate the protean functions of proteases in cancer biology.