Programmed Morphogenesis: Methods and Protocols

ISBN-13: 9781071611760 / Angielski / Miękka / 2021

Ebrahimkhani, Mo R.

Opis

This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as in vivo therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges.

Spis treści

Part I: Understanding Signaling Events and Patterns

 

1. TASBE Image Analytics: A Processing Pipeline for Quantifying Cell Organization from Fluorescent Microscopy

            Nicholas Walczak, Jacob Beal, Jesse Tordoff, and Ron Weiss

 

2. Neighborhood Impact Factor to Study Cell-Fate Decision-Making in Cellular Communities

            Shay Carter, Joshua Hislop, Joshua Hsu, Jeremy J. Velazquez, and Mo R. Ebrahimkhani

 

3. A Quantitative Lineage-Tracing Approach to Understand Morphogenesis in Gut

            Svetlana Ulyanchenko and Jordi Guiu

 

Part II: Programming Signaling Events and Patterns

 

4. Reconstitution of Morphogen Signaling Gradients in Cultured Cells

            Julia S. Kim, Michael Pineda, and Pulin Li

 

5. Engineering Shape-Controlled Microtissues on Compliant Hydrogels with Tunable Rigidity and Extracellular Matrix Ligands

            Megan L. Rexius-Hall, Nethika R. Ariyasinghe, and Megan L. McCain

 

6. Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives

            Thomas Richardson, Shibin Mathew, Connor Wiegand, Kevin Pietz, Joseph Candiello, K Ravikumar, and Ipsita Banerjee

 

7. Rewiring Endogenous Bioelectric Circuits in the Xenopus laevis Embryo Model

            Vasilios Nanos and Michael Levin

 

8. Engineering the Spatiotemporal Mosaic Self-Patterning of Pluripotent Stem Cells

            Ashley R.G. Libby, David A. Joy, and Todd C. McDevitt

 

Part III: Early Developmental Engineering

 

9. Fate-Patterning of 2D Gastruloids and Ectodermal Colonies Using Micropatterned Human Pluripotent Stem Cells

            George Britton, Sapna Chhabra, Joseph Massey, and Aryeh Warmflash

 

10. Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos

            Kerim Anlas, Peter Baillie-Johnson, Krisztina Arató, David A. Turner, and Vikas Trivedi

 

Part IV: Organoids, Tissue Barriers, and Disease Models

 

11. A Synergistic Engineering Approach to Build Human Brain Spheroids

            Djuna von Maydell and Mehdi Jorfi

 

12. Directed Differentiation of Human Pluripotent Stem Cells for the Generation of High-Order Kidney Organoids

            Idoia Lucía Selfa, Maria Gallo, Núria Montserrat, and Elena Garreta

 

13. Methods for Controlled Induction of Singular Rosette Cytoarchitecture within Human Pluripotent Stem Cell-Derived Neural Multicellular Assemblies

            Alireza Aghayee and Randolph Ashton

 

14. 3D Self-Organized Human Blood-Brain Barrier in a Microfluidic Chip

            Marco Campisi, Sei Hien Lim, Valeria Chiono, and Roger Kamm

 

15. Modeling the Complexity of the Metastatic Niche Ex Vivo

            Amanda M. Clark

 

16. Fabrication Method of a High-Density Co-Culture Tumor-Stroma Platform to Study Cancer Progression

            Harpinder Saini and Mehdi Nikkhah

 

Part V: In Vivo Therapeutic Applications

 

17. A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization

            Amy E. Emerson, Emily M. Slaby, and Jessica D. Weaver

 

18. High Throughput Production of Platelet-Like Particles

            Kylie M. Persson, Pauline V. Kneller, Mark W. Livingston, Lucas M. Bush, and Tara L. Deans