Progenitor Cells: Methods and Protocols

ISBN-13: 9781493996308 / Angielski / Twarda / 2019 / 289 str.

Mugdha V. Joglekar; Anandwardhan A. Hardikar

Spis treści

Dedication…
Preface…
Table of Contents…
Contributing Authors…

1. Isolation and Molecular Characterization of Progenitor Cells from Human Umbilical Cord
Umesh Goyal, Ankita Sen, and Malancha Ta

2. Isolation and Characterization of Extracellular Vesicles from Mesenchymal Stromal Cells
Sally Yunsun Kim, Thanh Huyen Phan, Christina Limantoro, Bill Kalionis, and Wojciech Chrzanowski

3. Assessment of β-Cell Replication in Isolated Rat Islets of Langerhans
Louise T. Dalgaard

4. Droplet Digital PCR for Measuring Absolute Copies of Gene Transcripts in Human Islet-Derived Progenitor Cells
Cody-Lee Maynard, Wilson K. M. Wong, Anandwardhan A. Hardikar, and Mugdha V. Joglekar

5. Isolation and In Vitro Culture of Human Gut Progenitor Cells
Jessica Bruce, Gerard E. Kaiko, and Simon Keely

6. Isolation and Characterization of Colony-Forming Progenitor Cells from Adult Pancreas
Janine C. Quijano, Jacob R. Tremblay, Jeffrey Rawson, and Hsun Teresa Ku

7. A Novel Gene Delivery Approach Using Metal Organic Frameworks in Human Islet-Derived Progenitor Cells
Arpita Poddar, Mugdha V. Joglekar, Anandwardhan A. Hardikar, and Ravi Shukla

8. Encapsulation and Transplantation of Pancreatic Progenitor Cells
Luke Carroll, Auvro R. Mridha, and Bernard E. Tuch

9. Differentiation of Urothelium from Mouse Embryonic Stem Cells in Chemically-Defined Conditions
Badwi B. Boumelhem, Stuart T. Fraser, and Stephen J. Assinder

10. Isolation and Characterization of Progenitor Cells from Human Adipose Tissue
Nitya Shree and Ramesh Bhonde

11. Identification and Analysis of Mouse Erythroid Progenitor Cells
Chanukya K. Colonne, Jia Hao Yeo, Campbell V. McKenzie, and Stuart T. Fraser

12. Single Cell Assays Using Hematopoietic Stem and Progenitor Cells
Ashwini S. Hinge and Marie Dominique Filippi

13. Isolation and Characterization of Cardiac Progenitor Cells
Parul Dixit and Rajesh G. Katare

14. Mitochondrial Assays Using Cardiac Stem Cells
Ayeshah A. Rosdah, Lea M. D. Delbridge, and Shiang Y. Lim

15. Two-Dimensional Electrophoresis and Mass Spectrometry for Protein Identification
Amaresh K. Ranjan and Anil Gulati

16. High-Efficiency Lentiviral Gene-Modification of Primary Murine Bone-Marrow Mesenchymal Stem Cells
Dario Gerace, Binhai Ren, Rosetta Martiniello-Wilks, and Ann M. Simpson

17. Bone Marrow-Derived Progenitor Cells Mediate Immune Cell Regulation
Kisha N. Sivanathan and Patrick T. Coates

18. Flow Cytometry and Cell Sorting Using Hematopoietic Progenitor Cells
Sangeetha Vadakke-Madathil, Lalita S. Limaye, Vaijayanti P. Kale, and Hina W. Chaudhry

19. Isolation of Epidermal Progenitor Cells from Rat Tympanic Membrane
Lawrence J. Liew, Allen Y. Wang, and Rodney J. Dilley

20. Measurement of Store-Operated Calcium Entry in Human Neural Cells: From Precursors to Differentiated Neurons
Renjitha Gopurappilly, Bipan Kumar Deb, Pragnya Chakraborty, and Gaiti Hasan

21. Embryoid Body Differentiation of Mouse Embryonic Stem Cells to Neurectoderm and Neural Progenitors
Rachel Shparberg, Hannah Glover, and Michael B. Morris

Blurb

This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells. The chapters in this book cover topics such as isolating progenitor cells from sources including adipose tissue, blood, bone marrow, ear, gut, heart, pancreatic islets, and Whirton’s jelly; deriving neural, pancreatic, and urothelial precursors from the embryonic stem cells; and culturing isolated islets in vitro to generate progenitor cells via epithelial-to-mesenchymal transition. This book also delves into digital droplet PCR; flow cytometry and cell sorting; mitochondrial assays; calcium ratiometric signaling; and 2D gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Progenitor Cells: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving and diverse field.