Probing Protein Adsorption on Model Surfaces

ISBN-13: 9783639100594 / Angielski / Miękka / 2008 / 124 str.

Francis Nsiah; Mark T. McDermott

Opis

The ability to control nonspecific protein adsorption at interfaces is a key issue in the design of biosensors, assays, chromatographic separations and implant materials. The research presented in this manuscript focuses on the effect of surface composition on the properties of adsorbed protein layers and the probing of protein-protein interactions both on adsorbed surfaces and in solution. Monolayers of alkanethiols on gold formed from solution self-assembly and microfluidic patterning using poly(dimethyl) siloxane (PDMS) served as platform for protein ad­sorption. We are employed surface plasma resonance (SPR) imaging and infrared reflection absorption spectroscopy (IRRAS) to probe the con­formation and orientation of adsorbed proteins, as well as, the characterization of protein layers.

O autorze

Francis Nsiah
After graduating in Chemistry from University of Cape Coast,
Ghana, Dr. Nsiah went on to University of Alberta, Canada, where
he obtained his MSc and PhD by carrying out research into surface
characterization of thin films and protein-protein interactions.

Mark T. McDermott is Chemistry Professor at the University of
Alberta, Canada.