Preeclampsia: Methods and Protocols

ISBN-13: 9781493985043 / Angielski / Miękka / 2018 / 355 str.

Padma Murthi; Cathy Vaillancourt

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. Diagnostic Imaging: Ultrasound
Stefan C. Kane, Su Lynn Khong, and Fabricio da Silva Costa

2. Biomarker Immunoassays in the Diagnosis of Pre-Eclampsia: Calculating the sFlt1:PlGF Ratio Using the Cobas e 411 Analyzer
Carin Black and Fabricio da Silva Costa

3. Assessing the Circulating Placental Specific Anti-Angiogenic Protein sFLT-1 e15a in Pre-Eclampsia
Kirsten Palmer

4. Role of Activin A in the Pathogenesis of Endothelial Cell Dysfunction in Pre-Eclampsia
Sebastian R. Hobson, Rebecca Lim, Joanne C. Mockler, Seshini Gurusinghe, and Euan M. Wallace

5. Genetic Approaches in Pre-Eclampsia
Hannah E. J. Yong, Padma Murthi, Shaun P. Brennecke, and Eric K. Moses

6. Epigenetics and Pre-Eclampsia: Programming of Future Outcomes
Alberto Borges Peixoto, Liliam Cristine Rolo, Luciano Marcondes Machado Nardozza, and Edward Araujo Júnior

7. Inflammatory and Immune System Markers
Kelly J. McKelvey, Gaayathri Ariyakumar, and Sharon A. McCracken

8. Methods to Enrich Exosomes from Conditioned Media and Biological Fluids
Shayna Sharma, Katherin Scholz-Romero, Gregory E. Rice, and Carlos Salomon

9. Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants
Mancy Tong and Larry W. Chamley

10. Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation
Andrew Lai, Omar Elfeky, Gregory E. Rice, and Carlos Salomon

11. Proteomics Method to Identification of Protein Profiles in Exosomes
Vyjayanthi Kinhal, Andrew Lai, Zarin Nuzhat, Ramkumar Menon, Gregory E. Rice, and Carlos Salomon

12. Harvesting and Characterization of Syncytial Nuclear Aggregates following Culture of First Trimester Human Placental Explants
Priyadarshini Pantham and Larry W. Chamley

13. Use of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion Index
Severine A. Degrelle and Thierry Fournier

14. Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of their Off-Target Effects
Paul Brownbill, Neil Sebire, Erin V. McGillick, Stacey Ellery, and Padma Murthi

15. Immunohistological Techniques
Evangelina Capobianco and Nora Martinez

16. Using a Next Generation Sequencing Approach to Profile MicroRNAs from Human Origin
Dominic Guanzon, Juvita Delancy Iljas, Gregory E. Rice, and Carlos Salomon

17. Isolation and Purification of Villous Cytotrophoblast Cells from Term Human Placenta
Hélène Clabault, Laetitia Laurent, J. Thomas Sanderson, and Cathy Vaillancourt

18. Analyzing Trophoblast Function Using Cell-Based Assays
Katie L. Powell and Anthony W. Ashton

19. Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and decidua basalis
Gina D. Kusuma, Mohamed H. Abumaree, Mark D. Pertile, and Bill Kalionis

20. An Electrical Impedance-Based Assay to Examine Functions of Various Placental Cell Types In Vitro
Tejasvy Chollangi, Hélène Clabault, Andrée-Anne Hudon Thibeault, Hannah E. J. Yong, Shagun Narula, Ellen Menkhorst, J. Thomas Sanderson, Cathy Vaillancourt, and Padma Murthi

21. In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases
Lucas Sagrillo-Fagundes, Laetitia Laurent, and Cathy Vaillancourt

22. Measurement of Oxidative Stress-Mitochondrial Function Using the Seahorse System
Dilys TH Leung and Simon Chu

23. Co-Culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-Placental Interactions: Focus on Estrogen Biosynthesis
Andrée-Anne Hudon Thibeault, J. Thomas Sanderson, and Cathy Vaillancourt

24. Placental Lipid Transport
Evemie Dubé, Guillaume Desparois, and Julie Lafond

25. EG-VEGF Maintenance over Early Gestation to Develop a Pregnancy Induced Hypertensive Animal Model
Deborah Reynaud, Frédéric Sergent, Roland Abi Nahed, Sophie Brouillet, Mohamed Benharouga, and Nadia Alfaidy

26. Real-Time Blood Pressure Recording Using Radiotelemetry in a Rat Model of Pre-Eclampsia
Bryan Leaw, Seshi Gurusinghe, Rebecca Lim, and Euan M. Wallace

27. Phase I Pilot Clinical Trial of Antenatal Maternally Administered Melatonin to Decrease the Level of Oxidative Stress in Human Pregnancies Affected by Pre-Eclampsia
Sebastian R. Hobson, Rebecca Lim, and Euan M. Wallace

28. A Randomized Double-Blinded Placebo-Controlled Intervention Trial of Melatonin for the Prevention of Pre-Eclampsia in Moderate- and High-Risk Women: The MELPOP Trial
Sebastian R. Hobson, Euan M. Wallace, John C. Kingdom, and Ryan J. Hodges

Blurb

This volume presents the latest developments and techniques used to study the physiopathology, diagnosis, and treatment of pre-eclampsia. Written for clinicians, obstetricians, basic scientists, researchers, and students the chapters in this book provide methods to study placental function using in vitro and ex vivo model systems, comprehensive genetic analysis of pre-eclampsia, identifying critical angiogenic factors associated with the development of pre-eclampsia, and controlled experiments that investigate potential therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls, as well as a description of the limitations and advantages of the described techniques.

Cutting-edge and comprehensive, Pre-Eclampsia: Methods and Protocols is a valuable resource for anyone interested in learning more about the critical role of the placenta in the maternal-fetal relationship.