Practical Guide to Chip-Seq Data Analysis

ISBN-13: 9781032241760 / Angielski / Miękka / 2021 / 112 str.

Borbala Mifsud; Kathi Zarnack; Anaïs F. Bardet

Opis

Chromatin immunoprecipitation sequencing (ChIP-seq) is amongst the most widely used methods in molecular biology. This practical book guides experimental biologists and bioinformaticians through the points one needs to consider when designing such a study and the analysis steps from quality control to visualisation.

Spis treści

Chapter 1 Introduction to ChIP-seq

Chapter 2 Getting Started

Chapter 3 General Quality Control

Chapter 4 Genomic Alignment

Chapter 5 ChIP-seq-specific Quality Control

Chapter 6 Peak Calling

Chapter 7 Data visualisation

Chapter 8 Comparative Analysis

Chapter 9 Downstream Analyses

O autorze

Borbala Mifsud is an assistant professor in Epigenomics at the Hamad Bin Khalifa University, Doha, Qatar. She is a computational biologist with a background in molecular biology and works on 3D chromatin conformation and the integration of epigenomic data.

Kathi Zarnack is a principal investigator in Computational RNA Biology at the Buchmann Institute for Molecular Life Sciences (BMLS), Goethe University Frankfurt, Germany. She is a computational biologist with a background in molecular biology and broad experience in analysing high-throughput sequencing data.

Anaïs F Bardet is a tenured researcher at the National Center for Scientific Research (CNRS) at the University of Strasbourg, France. She is a computational biologist and develops projects exploring the regulation of transcription factor binding.