Practical Fluorescence Spectroscopy

ISBN-13: 9781439821695 / Angielski / Twarda / 2019 / 772 str.

Zygmunt Gryczynski; Ignacy Gryczynski

Opis

Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence.

Spis treści

Preface. Theory of Light and Light Interaction with Matter. Experimental Basics. Steady State Experiments: Trasmission/Absorption. Fluorescence: Steady-State Phenomena. Steady-State Fluorescence: Applications. Steady-State Fluorescence Polarization: Anisotropy. Fluorescence: Time-Resolved Phenomena. Advanced Experiments.