Practical Flow Cytometry in Ha

ISBN-13: 9781118747032 / Angielski / Twarda / 2015 / 416 str.

Mike Leach; Mark Drummond; Allyson Doig

Opis

The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors' institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May-GrUnwald-Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples

• Provides a practical, example-based resource for flow cytometry
• Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care
• Includes both malignant and benign conditions
• Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207)

Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.

A companion book to Practical Flow Cytometry in Haematology Diagnosis. This title contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution.

Spis treści

Preface, vii

Acknowledgement, ix

List of Abbreviations, xi

Technical Notes, xv

Laboratory Values, xix

Case 1 1

Case 2 6

Case 3 11

Case 4 15

Case 5 18

Case 6 21

Case 7 24

Case 8 27

Case 9 31

Case 10 35

Case 11 39

Case 12 43

Case 13 46

Case 14 50

Case 15 54

Case 16 59

Case 17 62

Case 18 65

Case 19 68

Case 20 70

Case 21 74

Case 22 77

Case 23 80

Case 24 82

Case 25 87

Case 26 90

Case 27 93

Case 28 95

Case 29 100

Case 30 104

Case 31 106

Case 32 110

Case 33 114

Case 34 117

Case 35 122

Case 36 126

Case 37 129

Case 38 132

Case 39 136

Case 40 140

Case 41 143

Case 42 146

Case 43 151

Case 44 154

Case 45 159

Case 46 163

Case 47 166

Case 48 168

Case 49 172

Case 50 177

Case 51 180

Case 52 183

Case 53 186

Case 54 189

Case 55 193

Case 56 196

Case 57 201

Case 58 206

Case 59 210

Case 60 213

Case 61 216

Case 62 218

Case 63 224

Case 64 227

Case 65 232

Case 66 236

Case 67 240

Case 68 244

Case 69 249

Case 70 253

Case 71 256

Case 72 260

Case 73 266

Case 74 269

Case 75 274

Case 76 276

Case 77 281

Case 78 284

Case 79 289

Case 80 292

Case 81 297

Case 82 300

Case 83 306

Case 84 310

Case 85 315

Case 86 319

Case 87 321

Case 88 325

Case 89 327

Case 90 330

Case 91 334

Case 92 338

Case 93 342

Case 94 347

Case 95 351

Case 96 355

Case 97 359

Case 98 365

Case 99 370

Case 100 375

Antibodies Used in Immunohistochemistry Studies, 381

Flow Cytometry Antibodies, 386

Molecular Terminology, 389

Classification of Cases According to Diagnosis, 390

Index, 391

O autorze

Mike Leach FRCP, FRCPath
Consultant Haematologist and Honorary Senior Lecturer, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK

Mark Drummond PhD, FRCPath Consultant Haematologist and Honorary Senior Lecturer, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK

Allyson Doig MSc, FIBMS Haemato–Oncology Laboratory Manager, Haematology Laboratories Gartnavel General Hospital, Glasgow, UK

Pam McKay FRCP FRCPath Consultant Haematologist, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK

Bob Jackson MB MRCPI FRCPath Haematopathology Laboratories, Southern General Hospital, Glasgow, UK

Barbara J. Bain MB BS, FRACP, FRCPath, Professor of Diagnostic Haematology, St Mary s Hospital Campus of Imperial College Faculty of Medicine, London and Honorary Consultant Haematologist, St Mary s Hospital, London, UK

Blurb

The analysis of blood, bone marrow and tissue fluid specimens requires a multi–faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis.

This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis.

The reader will review May Grünwald Giemsa (MGG)–stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)–stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference.

Practical Flow Cytometry in Haematology: 100 Worked Examples:

– Provides a practical, example–based resource for flow cytometry
– Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care
– Includes both malignant and benign conditions
– Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207)

Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.