Post-Genomic Cardiology

ISBN-13: 9780124045996 / Angielski / Twarda / 2014 / 944 str.

Jose Marin-Garcia

Opis

In this second edition of Post-Genomic Cardiology, developing and new technologies such as translational genomics, next generation sequencing (NGS), bioinformatics, and systems biology in molecular cardiology are assessed in light of their therapeutic potential. As new methods of mutation screening emerge, both for the genome and for the "epigenome," comprehensive understanding of the many mutations that underlie cardiovascular diseases and adverse drug reactions is within our reach. This book, written by respected cardiologist Jose Marin-Garcia, features discussion on the Hap-Map: the largest international effort to date aiming to define the differences between our individual genomes. This unique reference further reviews and investigates genome sequences from our evolutionary relatives that could help us decipher the signals of genes, and offers a comprehensive and critical evaluation of regulatory elements from the complicated network of the background DNA.

• Offers updated discussion of cutting-edge molecular techniques including new genomic sequencing / NGS / Hap-Map / bioinformatics / systems biology approaches
• Analyzes mitochondria dynamics and their role in cardiac dysfunction, up-to-date analysis of cardio-protection, and cardio-metabolic syndrome
• Presents recent translational studies, gene therapy, transplantation of stem cells, and pharmacological treatments in CVDs

Recenzje

"This Second edition of Post-Genomic Cardiology (PCG) deals with what promises to be the cardiology of the future. As the role of genetic screening in cardiology is strengthened, and as research on the multiple signaling pathways involved in cardiac organogenesis and pathology progresses, this 2nd edition of PCG further attempts to integrate known facts with what is still developing and with what is slowly becoming known. New areas of interest to cardiologists and researchers include systems biology, the constructive cycle of computational model building, and experimental verification. These are capable of providing the input necessary for exciting new discoveries, including the management of diseases in a "personalized” way. The book is fascinating because it introduces a field that no one with a minimum amount of scientific interest will be able to avoid in the very near future. Extensively referenced, and thoroughly researched, this book presents well-written and organized chapters, each with a summary of the important points discussed, followed by exhaustive bibliographies. A detailed glossary clearly defines the many acronyms used throughout the book. Unquestionably, this volume will be of great interest and use to clinical and research cardiologists, pharmacologists and genetics investigators; and to students of basic sciences. Dr. Marin-Garcia - author and editor of this 2nd edition of PGC has done an outstanding job of completing this challenging task." --Dr. Daniel Shindler

"This is a great book." --Dr. A. Bayes de Luna

"Seven years ago, I was given the opportunity to review the first edition of this book, which was published in 2007. Dr. José Marín-García has updated and revised a new edition of that work. In these 7 years, we have been witnesses to the great surge in research into the genetic and molecular bases of cardiovascular disease. The use of ''omics'' has contributed enormously to this advance in the existing knowledge. The study of the genetic bases of diseases, building on linkage studies, genome-wide association studies, and genome sequencing (genomics), together with the new methods for studying gene expression (transcriptomics) and its regulation (epigenomics), advances in the quantification of proteins (proteo- mics) and their metabolites (metabolomics), the study of the molecular mechanisms that take place within the cell, such as signaling pathways and cascades, cell reprogramming, and the integration of all this information through the systems biology and the bioinformatics, have contributed to these advances. In this book, the author presents an ambitious review of all these aspects, focusing not only on genomic aspects, but on post-genomic aspects and their contribution to the current knowledge of the molecular bases of heart disease as well.

As I mentioned in my review of the earlier edition, the fact that the book was written by a single author, with the participation of three close associates, lends consistency and uniformity to the different chapters and facilitates their reading. Each chapter finishes with a highly useful summary that presents the main conclusions and the most important messages. The majority of the chapters provides a very extensive list of references (updated as of 2012), which facilitates the consultation of the original sources of the information. There are many tables summarizing the data and, in this new edition, the quality of the figures and graphs has improved quite notably.

The current edition has 11 well differentiated sections, each of which is divided into several chapters (between 1 and 5) that deal with different aspects related to the genomics of heart disease. With respect to the earlier edition, the contents have been updated and include new sections or chapters that are mainly related to the relevance of epigenetics and the mitochondrion to heart disease.

Section I offers a general introduction to the biochemical, cellular, and molecular functions of the heart under normal conditions. This section includes 3 chapters, the first 2 of which have been extensively rewritten. The 3 chapters introduce and review basic concepts related to gene structure, the processes of transcription and protein synthesis, and the cell cycle. The second chapter presents the methods for studying cardiac function at the molecular level, focusing on the different ''omics''. The last chapter of the section provides a review of the cell signaling pathways that regulate the growth, proliferation, and function of different cardiac cell lines. The author has updated the review of cell receptors and mitochondrial signaling, but the review of the intracellular pathways (adenyl cyclase, phospholipase, mitogen-activated protein [MAP] kinase, protein kinase, etc.), calcium signaling, and signals for cell cycle regulation, proliferation, and apoptosis has not been updated to any great extent in this second edition.

In section II, which is completely new, the author presents an excellent review, in 2 chapters, of the molecular mechanisms involved in embryonic heart development. In the first, he reviews the different signaling pathways and certain epigenetic factors that influence and regulate cell reprogramming in normal heart development; the second describes the known molecular changes that explain different congenital heart diseases.

Section III is the most extensive, with 5 chapters devoted to the analysis of arteriosclerosis, ischemic heart disease, angiogenesis, and hypertension. The first chapter describes the molecular mechanisms of arteriosclerosis and the second, the genetic bases of ischemic heart disease. In this second chapter, considerable weight is still given to evidence (often debatable) from linkage studies in family groups and from studies based on candidate genes. ...

Sections IV and V have also been extensively updated and restructured, and focus on the study of myocarditis, cardiomyopa- thies, and heart failure. They describe the genes and signaling pathways involved in cardiomyopathies (hypertrophic, idiopathic dilated, and restrictive cardiomyopathy and arrhythmogenic right ventricular dysplasia), in the cardiac response to inflammation and infection, and in the development of heart failure in patients with ischemic heart disease or hypertension. In this respect, the author has updated the content concerning ventricular remodeling, apoptosis and oxidative stress, and mitochondrial function in heart failure.

The book also reviews the molecular bases of arrhythmias, describing the genes involved in different channelopathies and in atrial fibrillation. The author presents and describes certain mutations than can be associated with a poorer prognosis in several heart diseases, although he admits that there are still many gaps in the current knowledge and, thus, cannot support their systematic utilization, and considers it necessary to continue to investigate the molecular mechanisms that regulate the different clinical presentations of a given genetic mutation.

Section VIII has only 1 chapter, which analyzes aspects related to sex differences in the context of cardiovascular diseases.

The last 3 sections are very new and provide an exhaustive presentation of aspects related to the aging of the heart and cardiovascular system (3 chapters), the epigenetic mechanisms related to cardiovascular diseases, and possible new therapeutic horizons that all this knowledge is opening along the path toward personalized cardiovascular medicine.

In conclusion, as I commented with regard to the previous edition, this is a highly interesting book that focuses on molecular mechanisms related to different heart diseases, which undoubtedly can be of great utility to cardiologists and researchers in different areas (pharmacology, basic research, clinical practice, epidemiology) who are interested in these mechanisms." --Roberto Elosua, Epidemiologia y Genetica Cardiovascular, Instituto Hospital del Mar de Investigaciones Medicas, Barcelona, Spain

Spis treści

Section I: Post-Genomic Cardiology 1. Introduction to the Molecular Biology of the Cell2. Methodologies in the Era of Cardiovascular "Omics” 3. Post-Genomics Cardiovascular Signaling Pathways

Section II: Pediatric Cardiology in the Post-Genomic Era 4. Molecular Determinants of Cardiac Development 5. Molecular Determinants of Congenital Heart Disease

Section III: Post-Genomic Assessment of Coronary Artery Disease, Angiogenesis, and Hypertension 6. Molecular Determinants of Atherosclerosis7. Molecular Genetics of Coronary Artery Disease and Myocardial Infarction 8. Cellular and Molecular Aspects of Cardioprotection 9. Molecular Determinants of Cardiac Neovascularization 10. Post-Genomics Appraisal of Systemic and Pulmonary Hypertension

Section IV: Post-Genomic Analysis of the Myocardium 11. Post-Genomic Appraisal of Myocarditis 12. Cardiomyopathies: A Comparative Analysis of Phenotypes and Genotypes

Section V: Heart Failure, Cell Death, and Mitochondria Dynamics 13. Gene Profiling, Energy Metabolism, and Remodeling of the Failing Heart14. Oxidative Stress and Cell Death in Cardiovascular Disease: A Post-Genomic Appraisal 15. The Emerging Role of Mitochondrial Dynamics in Cardiovascular Disease

Section VI: Molecular and Genetic Analysis of Metabolic Disorders 16. Post-Genomic Analysis of Fatty Acid and Glucose Metabolism in Cardiovascular Disease

Section VII: Molecular Genetics of Dysrhythmias 17. Post-Genomic Analysis of Dysrhythmias and Sudden Death

Section VIII: Genes, Gender, and Epigenetics 18. Gender and Cardiovascular Disease in the Post-Genomic Era

Section IX: Aging and the Cardiovascular System 19. The Aging Heart: A Post-Genomic Appraisal 20. Targeted Strategies to Fight Cardiac Aging21. Aging and the Cardiovascular System: The Road Ahead

Section X: Genetics, Epigenetics, and New Approaches to Treatment 22. Epigenetics and Cardiovascular Disease23. Gene- and Cell-Based Therapy for Cardiovascular Disease

Section XI: Looking to the Future24. The Path to Personalized Cardiovascular Medicine

O autorze

Dr.José Marín-García, highly respected cardiologist, is the current Director of Molecular Cardiology and Neuromuscular Institute in Highland Park, NJ. He has 189 listed publications and an H-index of 30. He has written and edited Mitochondria and the heart (2005), Aging and the Heart (2008), Signaling in the Heart (2008). Heart Failure (2010), Post-Genomic Cardiology (2007), and Mitochondria and Their Role in Cardiovascular Disease (2012).