Positron Emission Tomography: Methods and Protocols

ISBN-13: 9781071634981 / Angielski

Timothy H. Witney; Adam J. Shuhendler

Spis treści

Part I: Radiochemistry

 

1. ¹¹C-Fixation Techniques

            Emily Murrell, Anton Lindberg, Armando Garcia, and Neil Vasdev

 

2. Enantioselective Synthesis of Carbon-11 Labeled Amino Acids and Peptides

            Aleksandra Pekošak, Albert D. Windhorst, and Alex J. Poot

 

3. Silicon Fluoride Acceptors (SiFA) for ¹⁸F-Radiolabeling: From Bench to Bedside

            Lexi Gower-Fry, Carmen Wängler, Peter Bartenstein, Leonie Beyer, Simon Lindner, Klaus Jurkschat, Björn Wängler, Justin J. Bailey, and Ralf Schirrmacher

 

4. Tandem Iridium-Catalyzed C-H Borylation/Copper-Mediated Radiofluorination of Aromatic C-H Bonds with [¹⁸F]TBAF

            Maria Morales, Sean Preshlock, Liam S. Sharninghausen, Jay. S. Wright, Allen F. Brooks, Melanie S. Sanford, and Peter. J.H. Scott

 

5. Preparation of [⁶⁸Ga]GaCl₃ Using a Cyclotron

            Melissa E. Rodnick, Carina Sollert, D. Christian Parr, Jens Frigell, Katherine Gagnon, and Peter J.H. Scott

 

6. Transition and Post-Transition Radiometals for PET Imaging and Radiotherapy

            Caterina Ramogida and Eric Price

 

7. Rapid Radiolabeling for Peptide Radiotracers

            Xiaowei Ma and Zhen Cheng

 

8. Methods for the Production of Radiolabeled Bioagents for ImmunoPET

            Alejandro Arroyo, Serge K. Lyashchenko, and Jason S. Lewis

 

9. Radiolabeled Antibodies for Immune Checkpoint PET in Preclinical Research

            Milou Boswinkel, Gerben M. Franssen, and Sandra Heskamp

 

10. Radiolabeled Affibody Molecules for PET Imaging

            Chiara Da Pieve and Gabriela Kramer-Marek

 

Part II: Models and Validation

 

11. Small Animal Multisubject PET/CT Workflow

            Alexia Kirby and Adam J. Shuhendler

 

12. The Basics of Visualizing, Analyzing, and Reporting Preclinical PET/CT Imaging Data

            Christopher J. Cawthorne, Alessia Volpe, and Gilbert O. Fruhwirth

 

13. Radiosynthesis of [¹⁸F]FPenM-C2Am: A PET Imaging Agent for Detecting Cell Death

            Flaviu Bulat, Kevin M. Brindle, and André A.A. Neves

 

14. Imaging the Tumor Antioxidant Response with [¹⁸F]FSPG PET

            Oskar Vilhelmsson Timmermand and Timothy H. Witney

 

15. Characterization and Validation of Radiotracer Kinetics Using the Langendorff Isolated Perfused Heart

            Friedrich Baark, Edward C.T. Waters, Thomas R. Eykyn, and Richard Southworth

 

16. Brain PET Imaging in Small Animals: Tracer Formulation, Data Acquisition, Image Reconstruction, and Data Analysis

            Hussein Bdair, Min Su Kang, Julie Ottoy, Arturo Aliaga, Peter Kunach, Thomas A. Singleton, Stephan Blinder, Jean-Paul Soucy, Marco Leyton, Pedro Rosa-Neto, and Alexey Kostikov

 

17. CRISPR/Cas for PET Reporter Gene Engineering

            Taemoon Chung, Joseph R. Merrill, and Scott K. Lyons

 

18. Genetic Engineering of Therapeutic Cells with the Sodium Iodide Symporter (NIS) to Enable Non-Invasive In Vivo Therapy Tracking

            Ben Grimsdell, Adeel Saleem, Alessia Volpe, and Gilbert O. Fruhwirth

 

19. Single-Cell PET Imaging and Tracking

            Kyung Oh Jung and Guillem Pratx

 

Part III: PET Technology

 

20. PET System Technology: Theoretical Aspects and Experimental Methodology

            Andrea Gonzalez-Montoro and Craig S. Levin

 

21. Practical Considerations for Total-Body PET Acquisition and Imaging

            Benjamin A. Spencer, Kristin McBride, Heather Hunt, Terry Jones, Simon R. Cherry, and Ramsey D. Badawi

 

22. Combined PET/MR: Where Anatomical Imaging Meets Cellular Function

            Drew R. DeBay and Kimberly D. Brewer

 

23. Quantitation of Oncologic Image Features for Radiomic Analyses in PET

            Travis L. Williams, Mithat Gonen, Rick Wray, Richard K. G. Do, and Amber L. Simpson

 

24. Multiplexing Autoradiography

            David Y. Lewis

 

25. Kinetic Modeling Methods in Preclinical Positron Emission Tomography Imaging

            Agne Knyzeliene, Robert Shaw, Viktoria Balogh, and Adriana A.S. Tavares

Blurb

This detailed volume explores key concepts and experimental design related to Positron Emission Tomography (PET) imaging that have revolutionized our understanding of human biology. The first part focuses on recent advances in radiotracer probe development to enable the detection of materials, from large macromolecules to complicated drug-like structures. The next section describes how key physiological and pathophysiological processes can be interrogated and quantifiably measured with this imaging technique. Finally, chapters examine important technological developments in the field that are revolutionizing the way these innovative PET probes are utilized in the clinic. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Positron Emission Tomography: Methods and Protocols serves as an ideal guide for researchers looking to use imaging to revolutionize the way we diagnose and treat disease.