Polymerase Chain Reaction

ISBN-13: 9786200091253 / Angielski / Miękka / 112 str.

M. Arif Khawaja; Nishant Singh; Kanika Sharma

Opis

Polymerase chain reaction (PCR) is a laboratory technique used to amplify DNA sequences. The method involves using short DNA sequences called primers to select the portion of the genome to be amplified. The temperature of the sample is repeatedly raised and lowered to help a DNA replication enzyme copy the target DNA sequence. The technique can produce a billion copies of the target sequence in just a few hours. PCR is a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Because DNA polymerase can add a nucleotide only onto a preexisting 3'-OH group, it needs a primer to which it can add the first nucleotide. This requirement makes it possible to delineate a specific region of template sequence that the researcher wants to amplify. At the end of the PCR reaction, the specific sequence will be accumulated in billions of copies (amplicons). PCR is used in many areas of biology and medicine, including molecular biology research, medical diagnostics, and even some branches of ecology.

O autorze

Khawaja, M. ArifI am a dental surgeon with Jammu and Kashmir Health Services since 2001. I have done my BDS from Government Dental College Srinagar in 1998. Currently I am pursuing MDS from DJ College of Dental Sciences and Research Modinagar Ghaziabad UP (India).