Polymer-Protein Conjugates: From Pegylation and Beyond

ISBN-13: 9780444640819 / Angielski / Miękka / 2019 / 512 str.

Gianfranco Pasut; Samuel Zalipsky

Spis treści

Introduction and General Aspects of the Technology 1. Evolution of Polymer Conjugation to proteins 2. PEGylated Proteins: A Rational Design for Mitigating Clearance Mechanisms and Altering Biodistribution 3. Overcoming barriers for tumor-targeted drug delivery: the power of macromolecular anticancer drugs with the EPR effect and the modulation of vascular physiology

Toxicity and Immunogenicity Studies and Clinical Stage Conjugate Products 4. PEG-protein conjugates: Non-clinical and clinical toxicity considerations 5. Immunological responses to PEGylated proteins: anti-PEG antibodies 6. Immunogenicity assessment of PEGylated proteins, Lonquex, a PEGylated G-CSF case study 7. PEG-Protein conjugates: regulatory requirements for characterization 8. PEGylation of human coagulation factor VIII and other plasma proteins 9. Pegvorhyaluronidase Alfa: A PEGylated Recombinant Human Hyaluronidase PH20 for the Treatment of Cancers That Accumulate Hyaluronan 10. Challenges in the analytical characterization of PEGylated asparaginases

Novel Site-Selective Methods of Conjugation 11. Glycan-targeted PEGylation for selective modification of proteins 12. Sialyltransferase mediated glycoPEGylation 13. Enzymatic approaches to new protein conjugates 14. Site-specific Polymer-protein conjugates by Cys mutation 15. Site-specific PEGylation of interferon beta-1b: from bench to clinic 16. Protein modification by bis-alkylation

Alternative non-PEG polymers and architectures 17. In vivo properties of therapeutic bioconjugates composed of proteins and architecturally/functionally complex polymers 18. Poly(2-oxazoline)-protein conjugates 19. Polysaccharides for protein and peptide conjugation 20. Polysialylation of human coagulation factor VIII