Polyamines: Methods and Protocols

ISBN-13: 9781493958399 / Angielski / Miękka / 2016 / 523 str.

Anthony E. Pegg; Robert A. Casero Jr

Opis

Recent findings have revolutionized the study of these ubiquitous cellular components, essential for growth and development. Here, leading researchers contribute an extensive collection of up-to-date laboratory techniques that will enhance polyamine study.

Spis treści

Part I: Introduction

 

1. Current Status of the Polyamine Research Field

            Anthony E. Pegg and Robert A. Casero, Jr.

 

Part II: Identification of Polyamine Metabolic Enzymes and Characterization of Polyamine-Regulated Genes and Ion Channels

 

2. Exploring Polyamine Biosynthetic Diversity through Comparative and Functional Genomics

            Anthony J. Michael

 

3. Characterization of Genes for Polyamine Modulon

            Kazuei Igarashi and Keiko Kashiwagi

 

4. Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines

            Lan Xiao and Jian-Ying Wang

 

5. Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extreme Thermophiles

            Tairo Oshima, Toshiyuki Moriya, and Yusuke Terui

 

6. Polyamine Block of Inwardly-Rectifying Potassium Channels

            Harley T. Kurata, Wayland W.L. Cheng, and Colin G. Nichols

 

Part III: Transgenic Models for Study of Polyamine Function

 

7. Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism

            David J. Feith

 

8. Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Lipid Metabolism

            Anne Uimari, Mervi T. Hyvönen, Eija Pirinen, and Leena Alhonen

 

9. Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine

            Xiaojing Wang and Anthony E. Pegg

 

Part IV: Enzymes Involved in Polyamine Catabolism and Post-Translational Protein Modification

 

10. A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Response and Disease

            Andrew C. Goodwin, Tracy R. Murray-Stewart, and Robert A. Casero, Jr.

 

11. Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases

            Panagiotis N. Moschou and Kalliopi A. Roubelakis-Angelakis

 

12. Assay of Deoxyhypusine Synthase Activity

            Edith C. Wolff, Seung Bum Lee, and Myung Hee Park

 

13. Assay of Deoxyhypusine Hydroxylase Activity

            Jong Hwan Park, Edith C. Wolff, and Myung Hee Park

 

Part V: Regulation of Polyamine Content

 

14. Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase

            Erin K. Willert, Lisa N. Kinch, and Margaret A. Phillips

 

15. Protocols for Studying Antizyme Expression and Function

            Noriyuki Murai, Yasuko Murakami, and Senya Matsufuji

 

16. Identification, Assay, and Functional Analysis of the Antizyme Inhibitor Family

            Chaim Kahana

 

17. Post-Transcriptional Regulation of Ornithine Decarboxylase

            Shannon L. Nowotarski, Sofia Origanti, and Lisa M. Shantz

 

Part VI: Polyamine Transport and Uptake

 

18. Identification and Assays of Polyamine Transport Systems in Escherichia coli and Saccharomyces cerevisiae

            Keiko Kashiwagi and Kazuei Igarashi

 

19. Genetic and Biochemical Analysis of Protozoal Polyamine Transporters

            Marie-Pierre Hasne and Buddy Ullman

 

20. Heparan Sulfate Proteoglycan-Mediated Polyamine Uptake

            Johanna Welch, Katrin Svensson, Paulina Kucharzewska, and Mattias Belting

 

21. Polyamine Transport Systems in Mammalian Cells and Tissues

            Takeshi Uemura and Eugene W. Gerner

 

22. Procedures to Evaluate the Importance of Dietary Polyamines

            Paul Acheampong, Mary J. Macleod, and Heather M. Wallace

 

Part VII: Polyamines and Disease

 

23. Determination of N¹,N¹²-Diacetylspermine in Urine: A Novel Tumor Marker

            Masao Kawakita, Kyoko Hiramatsu, Mari Yanagiya, Yosuke Doi, and Mieko Kosaka

 

24. Spermidine/spermine-N¹-Acetyltransferase in Kidney Ischemia Reperfusion Injury

            Kamyar Zahedi and Manoocher Soleimani

 

25. Use of Polyamine Metabolites as Markers for Stroke and Renal Failure

            Kazuei Igarashi and Keiko Kashiwagi

 

26. Methods to Evaluate Alterations in Polyamine Metabolism Caused by Helicobacter pylori Infection

            Alain P. Gobert, Rupesh Chaturvedi, and Keith T. Wilson

 

27. High-Resolution Capillary Gas Chromatography in Combination with Mass Spectrometry for Quantification of Three Major Polyamines in Post-Mortem Brain Cortex

            Gary Gang Chen, Laura M. Fiori, Orval A. Mamer, and Gustavo Turecki

 

28. Spermine Synthase Deficiency Resulting in X-Linked Intellectual Disability (Snyder-Robinson Syndrome)

            Charles E. Schwartz, Xaiojing Wang, Roger E. Stevenson, and Anthony E. Pegg

 

Part VIII: Chemistry and Analysis

 

29. Methylated Polyamines as Research Tools

            Alex R. Khomutov, Janne Weisell, Maxim A. Khomutov, Nikolay A. Grigorenko, Alina R. Simonian, Merja R. Häkkinen, Tuomo A. Keinänen, Mervi T. Hyvönen, Leena Alhonen, Sergey N. Kochetkov, and Jouko Vepsäläinen

 

30. Fluorescent Substrates for Polyamine Catabolic Enzymes and Transport

            Koichi Takao and Akira Shirahata

 

31. Use of Polyamine Derivatives as Selective Histone Deacetylase Inhibitors

            Patrick M. Woster

 

32. Measurement of Polyamine pK_a Values

            Ian S. Blagbrough, Abdelkader A. Metwally, and Andrew J. Geall

 

33. Polyamine Analysis by LC-MS

            Merja R. Häkkinen

Blurb

Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development.  In Polyamines: Methods and Protocols, leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study.  The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Polyamines: Methods and Protocols provides a key resource for all scientists pursuing the study of this dynamic and significant aspect of cellular biology.