Poliovirus: Methods and Protocols

ISBN-13: 9781493932917 / Angielski / Twarda / 2016 / 341 str.

Javier Martin

Opis

This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Poliovirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Spis treści

1. An Introduction to Poliovirus: Pathogenesis, Vaccination and the Endgame for Global Eradication

Philip D Minor

2. Poliovirus Laboratory Based Surveillance: An Overview

Syed Sohail Zahoor Zaidi, Humayun Asghar, Salmaan Sharif, and Muhammad Masroor Alam

3. Isolation and Characterisation of Enteroviruses from Clinical Samples

Soile Blomqvist and Merja Roivainen

4. Isolation and Characterisation of Poliovirus in Cell Culture Systems

Bruce R. Thorley and Jason A. Roberts

5. Molecular Characterization of Polio from Environmental Samples – ISSP, The Israeli Sewage Surveillance Protocol

Lester M. Shulman, Yossi Manor, Musa Hindiyeh, Danit Sofer, and Ella Mendelson

6. Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays

Glynis Dunn

7. A Transgenic Mouse Model of Poliomyelitis

Satoshi Koike and Noriyo Nagata

8. Standardized Methods for Detection of Poliovirus Antibodies

William C. Weldon, M. Steven Oberste, and Mark A. Pallansch

9. Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis,

Typing by PCR and Real-time RT-PCR

Cara C. Burns, David R. Kilpatrick, Jane C. Iber, Qi Chen, and Olen M. Kew

10. Isolation and Characterization of Vaccine-Derived Polioviruses, Relevance for the Global Polio Eradication Initiative

Wenbo Xu

and Yong Zhang

11. Phylogenetic Analysis of Poliovirus Sequences

Jaume Jorba

12. Generation of Infectious Poliovirus with Altered Genetic Information from Cloned cDNA

Erika Bujaki

13. A Rapid Method for Engineering Recombinant Polioviruses or Other Enteroviruses

Maël Bessaud, Isabelle Pelletier, Bruno Blondel, and Francis Delpeyroux

14. Methods to Monitor Molecular Consistency of Oral Polio Vaccine

Konstantin Chumakov

15. Methods for the Quality Control of Inactivated Poliovirus Vaccines

Thomas Wilton

16. Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays

Janny Westdijk, Larissa van der Maas, Rimko ten Have, and Gideon Kersten

17. Identification and Analysis of Antiviral Compounds against Poliovirus

Pieter Leyssen, David Franco, Aloys Tijsma, Céline Lacroix, Armando De Palma, and Johan Neyts

Blurb

This volume introduces the reader to the field of systems medicine by defining its terminology, and describing how established computational methods from bioinformatics and systems biology can be taken forward to establish integrative systems medicine approaches.  Systems Medicine provides an outlook on the role that systems medicine may or should play in various medical fields. Describing different facets of the systems medicine approach in action, including examples related to human metabolism, chronic obstructive pulmonary disease, the RNAs in biomedicine and regenerative stem cell medicine.  Chapters also introduce tools, resources and methodologies from bioinformatics and systems biology, and shows how to apply these in a systems medicine project. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, and discuss experimental and computational approaches, methods,  and tools that should be considered for a successful systems medicine project. Systems Medicine aims to motivate and provide guidance for collaborations across disciplines to tackle today's challenges related to human health and well-being.